Induction of Manganese-Containing Superoxide Dismutase in Anaerobic Escherichia coli by Diamide and 1,10-Phenanthroline: Sites of Transcriptional Regulation

Transcriptional regulation of the sodA gene, a member of the soxRS regulon encoding the manganese-containing superoxide dismutase (MnSOD; superoxide:superoxide oxidoreductase, EC 1.15.1.1) of Escherichia coli, was examined in a variety of regulatory mutants. Diamide, an oxidant that causes the anaer...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1993-03, Vol.90 (6), p.2310-2314
Main Authors: Privalle, Christopher T., Kong, Stephanie E., Fridovich, Irwin
Format: Article
Language:English
Subjects:
Anaerobic conditions
Bacteria
Biochemistry
Biological and medical sciences
Biosynthesis
Diamide - pharmacology
Enzyme Induction
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - enzymology
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Gels
Gene expression regulation
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Genes
Genes, Bacterial
Genetic loci
Glucosephosphate Dehydrogenase - metabolism
Iron
Isoenzymes - biosynthesis
Isoenzymes - genetics
Manganese - analysis
Manganese - metabolism
Molecular and cellular biology
Molecular genetics
Oxidation
Phenanthrolines - pharmacology
Regulon
Superoxide Dismutase - biosynthesis
Superoxide Dismutase - genetics
Superoxides
Transcription. Transcription factor. Splicing. Rna processing
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Summary:Transcriptional regulation of the sodA gene, a member of the soxRS regulon encoding the manganese-containing superoxide dismutase (MnSOD; superoxide:superoxide oxidoreductase, EC 1.15.1.1) of Escherichia coli, was examined in a variety of regulatory mutants. Diamide, an oxidant that causes the anaerobic biosynthesis of the MnSOD polypeptide and also facilitates insertion of manganese at the active site, was found to anaerobically induce MnSOD in both soxRS and fur arcA fnr strains. Metal chelating agents also caused anaerobic induction of MnSOD in a fur arcA fnr triple mutant; however, this induction of MnSOD and of glucose-6-phosphate dehydrogenase (G6PD) by 1,10-phenanthroline was dependent on an intact soxRS locus. A strain of E. coli bearing a fusion of the soxS promoter to lacZ was used to demonstrate that both diamide and 1,10-phenanthroline caused anaerobic activation of soxS transcription. These results indicate that (i) both diamide and 1,10-phenanthroline induce the soxRS regulon anaerobically by stimulation of soxS transcription; (ii) diamide, but not metal chelators, also induces MnSOD biosynthesis by a soxRS-independent mechanism, perhaps mediated by effects on fur, arcA, or fnr-mediated repression of sodA; and (iii) the soxRS locus contains a metal-binding component and is responsive to the redox status of the cell.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.90.6.2310