Complementation of a Yeast Cell Cycle Mutant by an Alfalfa cDNA Encoding a Protein Kinase Homologous to p34cdc2

The cdc2 protein kinase plays a central role in control of the eukaryotic cell cycle of animals and yeasts. We have isolated a cDNA clone (cdc2Ms) from alfalfa (Medicago sativa L.) that is homologous to the yeast cdc2/CDC28 genes. The encoded protein is 64% identical to the yeast and mammalian count...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1991-03, Vol.88 (5), p.1636-1640
Main Authors: Hirt, Heribert, Pay, Aniko, Gyorgyey, Janos, Bako, Laszlo, Nemeth, Kinga, Borge, Laszlo, Schweyen, Rudolf J., Heberle-Bors, Erwin, Dudits, Denes
Format: Article
Language:English
Subjects:
Alfalfa
Amino Acid Sequence
Amino acids
Base Sequence
Biological and medical sciences
CDC2 Protein Kinase - genetics
Cell Cycle
Complementary DNA
Cultured cells
Fundamental and applied biological sciences. Psychology
Generally accepted auditing standards
Genes. Genome
Genetic Complementation Test
Humans
Medicago sativa - enzymology
Medicago sativa - genetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nurses
Oligonucleotide Probes
Polymerase Chain Reaction
Protein Kinases - genetics
RNA
Schizosaccharomyces - genetics
Sequence Homology, Nucleic Acid
Yeasts
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Summary:The cdc2 protein kinase plays a central role in control of the eukaryotic cell cycle of animals and yeasts. We have isolated a cDNA clone (cdc2Ms) from alfalfa (Medicago sativa L.) that is homologous to the yeast cdc2/CDC28 genes. The encoded protein is 64% identical to the yeast and mammalian counterparts and shows all the prominent structural features known from these organisms. Antibody raised against a 16-amino acid synthetic peptide with crossreactivity against p34 proteins recognized a 34-kilodalton protein in extracts of alfalfa cells. When transferred into a fission yeast, the plant cdc2 homolog can complement a temperature-sensitive cdc2 mutant. Northern analysis revealed higher transcript levels in shoots and suspension cultures than in roots. In addition to the dominant transcript of 1.4 kilobases detected in the poly(A)+fraction, 2.5- and 1.2-kilobase transcripts were detected in total RNA preparations from shoots or somatic embryos. Suspension cultures that were induced to form somatic embryos by an auxin (2,4-dichlorophenoxyacetic acid) showed fluctuations in transcription pattern during the induction period and embryogenesis.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.88.5.1636