ISGF3, the Transcriptional Activator Induced by Interferon α, Consists of Multiple Interacting Polypeptide Chains

Interferon-stimulated gene factor 3 (ISGF3) is the ligand-dependent transcriptional activator that, in response to interferon treatment, is assembled in the cell cytoplasm, is translocated to the nucleus, and binds the consensus DNA site, the interferon-stimulated response element. We have purified...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1990-11, Vol.87 (21), p.8555-8559
Main Authors: Fu, Xin-Yuan, Kessler, Daniel S., Veals, Susan A., Levy, David E., Darnell, J. E.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Base Sequence
Biological and medical sciences
Chromatography
Chromatography, Affinity
Chromatography, Ion Exchange
Cytoplasm
DNA
DNA-Binding Proteins - isolation & purification
DNA-Binding Proteins - metabolism
Fundamental and applied biological sciences. Psychology
Gels
Genes
HeLa cells
HeLa Cells - drug effects
HeLa Cells - metabolism
Humans
Interferon Type I - pharmacology
Interferon-Stimulated Gene Factor 3
Interferon-Stimulated Gene Factor 3, gamma Subunit
Interferons
Macromolecular Substances
Miscellaneous
Molecular Sequence Data
Molecular Weight
Oligodeoxyribonucleotides
Oligonucleotides
Proteins
Receptors
Response elements
Transcription Factors - isolation & purification
Transcription Factors - metabolism
Transcription, Genetic
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Description
Summary:Interferon-stimulated gene factor 3 (ISGF3) is the ligand-dependent transcriptional activator that, in response to interferon treatment, is assembled in the cell cytoplasm, is translocated to the nucleus, and binds the consensus DNA site, the interferon-stimulated response element. We have purified ISGF3 and identified its constituent proteins: a DNA-binding protein of 48 kDa and three larger polypeptides (84, 91, and 113 kDa), which themselves do not have DNA-binding activity. The multisubunit structure of ISGF3 most likely reflects its participation in receiving a ligand-dependent signal, translocating to the nucleus, and binding to DNA to activate transcription.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.87.21.8555