Diagnosis of Chronic Myeloid and Acute Lymphocytic Leukemias by Detection of Leukemia-Specific mRNA Sequences Amplified in vitro

The Philadelphia chromosome is present in more than 95% of chronic myeloid leukemia patients and 13% of acute lymphocytic leukemia patients. The Philadelphia translocation, t(9;22), fuses the BCR and ABL genes resulting in the expression of leukemia-specific, chimeric BCR-ABL messenger RNAs. To faci...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-08, Vol.85 (15), p.5698-5702
Main Authors: Kawasaki, Ernest S., Clark, Steven S., Coyne, Mazie Y., Smith, Stephen D., Champlin, Richard, Witte, Owen N., McCormick, Frank P.
Format: Article
Language:English
Subjects:
550201 - Biochemistry- Tracer Techniques
BASIC BIOLOGICAL SCIENCES
Biological and medical sciences
BIOLOGICAL MARKERS
Cell Line
Cell lines
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
Chronic myeloid leukemia
DIAGNOSIS
DISEASES
DNA
DNA SEQUENCING
Exons
Gene Amplification
Hematologic and hematopoietic diseases
HEMIC DISEASES
Humans
IMMUNE SYSTEM DISEASES
Immunoassay
K562 cells
KARYOTYPE
Karyotyping
LEUKEMIA
Leukemia, Lymphoid - diagnosis
Leukemia, Lymphoid - genetics
Leukemia, Myeloid - diagnosis
Leukemia, Myeloid - genetics
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Medical sciences
MESSENGER-RNA
MOLECULAR STRUCTURE
MUTATIONS
NEOPLASMS
Nucleic Acid Hybridization
NUCLEIC ACIDS
Oligonucleotides
ORGANIC COMPOUNDS
PATIENTS
PHILADELPHIA CHROMOSOME
Polymerase chain reaction
RNA
RNA, Messenger - analysis
RNA, Messenger - immunology
RNA, Neoplasm - analysis
STRUCTURAL CHEMICAL ANALYSIS
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Description
Summary:The Philadelphia chromosome is present in more than 95% of chronic myeloid leukemia patients and 13% of acute lymphocytic leukemia patients. The Philadelphia translocation, t(9;22), fuses the BCR and ABL genes resulting in the expression of leukemia-specific, chimeric BCR-ABL messenger RNAs. To facilitate diagnosis of these leukemias, we have developed a method of amplifying and detecting only the unique mRNA sequences, using an extension of the polymerase chain reaction technique. Diagnosis of chronic myeloid and acute lymphocytic leukemias by this procedure is rapid, much more sensitive than existing protocols, and independent of the presence or absence of an identifiable Philadelphia chromosome.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.15.5698