Molecular Cloning of Human T-Cell Lymphotrophic Virus Type I-Like Proviral Genome from the Peripheral Lymphocyte DNA of a Patient with Chronic Neurologic Disorders

Human T-cell lymphotropic virus type 1 (HTLV-I), the etiologic agent of human T-cell leukemia, has recently been shown to be associated with neurologic disorders such as tropical spastic paraparesis, HTLV-associated myelopathy, and possibly with multiple sclerosis. In this communication, we have exa...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-05, Vol.85 (10), p.3599-3603
Main Authors: Reddy, E. Premkumar, Mettus, Richard V., DeFreitas, Elaine, Wroblewska, Zophia, Cisco, Maria, Koprowski, Hilary
Format: Article
Language:English
Subjects:
550201 - Biochemistry- Tracer Techniques
Adult
AIDS/HIV
AMINO ACIDS
ANIMAL CELLS
ANTIBODIES
B lymphocytes
BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
Cell lines
Chronic Disease
CLONING
Cloning, Molecular
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
Deltaretrovirus - genetics
Deltaretrovirus Infections - microbiology
DISEASES
DNA
DNA - blood
DNA - genetics
DNA HYBRIDIZATION
DNA probes
DNA Restriction Enzymes
DNA, Viral - genetics
DNA-CLONING
DRUGS
EVEN-ODD NUCLEI
Genes, Viral
GENETICS
Genomes
Haiti - ethnology
Human T lymphotropic virus 1
Humans
HYBRIDIZATION
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LYMPHOCYTES
Lymphocytes - microbiology
Male
MATERIALS
MESSENGER-RNA
METHIONINE
MICROORGANISMS
NERVOUS SYSTEM DISEASES
Nervous System Diseases - microbiology
NUCLEI
Nucleic Acid Hybridization
NUCLEIC ACIDS
Nucleotide Mapping
ONCOGENIC VIRUSES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PATIENTS
Proviruses - genetics
RADIOISOTOPES
RNA
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
T lymphocytes
United States
VIRUSES
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Description
Summary:Human T-cell lymphotropic virus type 1 (HTLV-I), the etiologic agent of human T-cell leukemia, has recently been shown to be associated with neurologic disorders such as tropical spastic paraparesis, HTLV-associated myelopathy, and possibly with multiple sclerosis. In this communication, we have examined one specific case of neurologic disorder that can be classified as multiple sclerosis or tropical spastic paraparesis. The patient suffering from chronic neurologic disorder was found to contain antibodies to HTLV-I envelope and gag proteins in his serum and cerebrospinal fluid. Lymphocytes from peripheral blood and cerebrospinal fluid of the patient were shown to express viral RNA sequences by in situ hybridization. Southern blot analysis of the patient lymphocyte DNA revealed the presence of HTLV-I-related sequences. Blot-hybridization analysis of the RNA from fresh peripheral lymphocytes stimulated with interleukin 2 revealed the presence of abundant amounts of genomic viral RNA with little or no subgenomic RNA. We have cloned the proviral genome from the DNA of the peripheral lymphocytes and determined its restriction map. This analysis shows that this proviral genome is very similar if not identical to that of the prototype HTLV-I genome.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.10.3599