Recombinational substrates designed to study recombination between unique and repetitive sequences in vivo

Three recombination events, reciprocal recombination, sister-chromatid recombination, and gene conversion, were studied using substrates designed in vitro. Each type of recombination event can be monitored at any chromosomal location. We have shown that sister-chromatid recombination is induced mito...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-09, Vol.84 (17), p.6215-6219
Main Authors: Fasullo, M.T, Davis, R.W
Format: Article
Language:English
Subjects:
Alleles
Base Sequence
Biological and medical sciences
Chromosomes
DNA
DNA, Fungal - genetics
Fundamental and applied biological sciences. Psychology
Gene Conversion
Genes
Genetic loci
GENETICA
GENETICS
GENETIQUE
Genic rearrangement. Recombination. Transposable element
Genomes
Mesylates
Molecular and cellular biology
Molecular genetics
Plasmids
RECOMBINACION
RECOMBINAISON
RECOMBINATION
Recombination, Genetic
Repetitive Sequences, Nucleic Acid
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
Sister Chromatid Exchange
Yeasts
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Summary:Three recombination events, reciprocal recombination, sister-chromatid recombination, and gene conversion, were studied using substrates designed in vitro. Each type of recombination event can be monitored at any chromosomal location. We have shown that sister-chromatid recombination is induced mitotically by DNA damaging agents, such as methyl methanesulfonate and γ -rays, but is decreased mitotically in strains defective in rad52. Reciprocal recombination by which circular plasmids integrate into the genome is unaffected by rad52 defective alleles and occurs by a different recombination pathway. Mechanisms are suggested by which gene conversion between sister chromatids can generate chromosome rearrangements.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.17.6215