The Molecular Cloning of a type II Regulatory Subunit of the cAMP-Dependent Protein Kinase from Rat Skeletal Muscle and Mouse Brain

A cDNA clone for a type II regulatory (R) subunit of the cAMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) was isolated from a rat skeletal muscle library using a specific 47-base oligonucleotide probe. The rat cDNA was 1.2 kilobases (kb) in length and contained an open rea...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-08, Vol.84 (15), p.5192-5196
Main Authors: Scott, John D., Glaccum, Moira B., Zoller, Mark J., Uhler, Michael D., Helfman, David M., McKnight, G. Stanley, Krebs, Edwin G.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Base Sequence
Biological and medical sciences
Biotechnology
brain
Brain - enzymology
Cattle
Cloning, Molecular
Complementary DNA
DNA
DNA Restriction Enzymes - metabolism
Fundamental and applied biological sciences. Psychology
Generally accepted auditing standards
Genetic engineering
Genetic technics
Macromolecular Substances
Messenger RNA
Methods. Procedures. Technologies
Mice
Molecular cloning
Muscles - enzymology
nucleotide sequence
Nucleotide sequences
Open reading frames
protein kinase
Protein Kinases - genetics
Rats
RNA
Skeletal muscle
Swine
Ungulates
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Summary:A cDNA clone for a type II regulatory (R) subunit of the cAMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) was isolated from a rat skeletal muscle library using a specific 47-base oligonucleotide probe. The rat cDNA was 1.2 kilobases (kb) in length and contained an open reading frame of 1.113 kb representing 92% of the coding region of the molecule. Nick-translated rat cDNA was then used to isolate a mouse RII cDNA clone from a brain library that contained an open reading frame of 1.143 kb. Because both cDNAs lacked complete coding sequences, the remainder of the RII coding region was obtained from a 15-kb mouse genomic clone. The mouse RII coding region contains 1.2 kb corresponding to a 400-amino acid protein of 51.141 kDa. The mouse cDNA hybridizes to two mRNA species, a 2.4-kb form that was only observed in testis and a 6.0-kb form found in a wide range of tissues, including testis.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.15.5192