Characterization of Nerve Growth Factor Receptor in Neural Crest Tumors Using Monoclonal Antibodies

The nerve growth factor (NGF) receptor was characterized by using a new series of anti-receptor monoclonal antibodies (MAbs). These MAbs (i) showed significantly greater reactivity with a melanoma cell line expressing higher levels of NGF receptor, (ii) inhibited the binding of125I-labeled NGF to it...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1984-11, Vol.81 (21), p.6681-6685
Main Authors: Ross, Alonzo H., Grob, Peter, Bothwell, Mark, Elder, David E., Ernst, Carolyn S., Marano, Nadia, Barbara F. D. Ghrist, Slemp, Catherine C., Herlyn, Meenhard, Atkinson, Barbara, Koprowski, Hilary
Format: Article
Language:English
Subjects:
Acinar cells
Affinity Labels
Animals
Antibodies, Monoclonal
Antigens
Antigens, Neoplasm - immunology
Cell Line
Cell lines
Cell membranes
Cross-Linking Reagents
Histocytochemistry
Humans
Immunoenzyme Techniques
Immunosorbent Techniques
man
Melanoma
Melanoma - metabolism
Mice
Mice, Inbred BALB C
monoclonal antibodies
nerve growth factor
Nerves
Neural crest
Neurons
PC12 cells
Receptors
Receptors, Cell Surface - immunology
Receptors, Cell Surface - metabolism
Receptors, Nerve Growth Factor
tumors
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Summary:The nerve growth factor (NGF) receptor was characterized by using a new series of anti-receptor monoclonal antibodies (MAbs). These MAbs (i) showed significantly greater reactivity with a melanoma cell line expressing higher levels of NGF receptor, (ii) inhibited the binding of125I-labeled NGF to its receptor, and (iii) immunoprecipitated both metabolically labeled and125I-labeled NGF affinity-labeled receptor. These experiments defined the receptor as a 75-kDa cell-surface protein. The NGF receptor was visualized by immunoperoxidase staining in tissue sections of human nevi, melanomas, neurofibromas, a pheochromocytoma, and peripheral nerves. Uniform staining of the cytoplasm suggests that, in addition to cell-surface NGF receptors, there is a population of intracellular receptors.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.81.21.6681