Synthesis of Epstein-Barr Virus after Activation of the Viral Genome in a "Virus-Negative" Human Lymphoblastoid Cell (Raji) Made Resistant to 5-bromodeoxyuridine

The Raji line of human lymphoblastoid cells, which does not show expression of Epstein-Barr virus, was made resistant to 5-bromodeoxyuridine. Within several weeks after removal of the drug, Epstein-Barr virus particles were detected in the cells. The sequence of virus appearance in the 5-bromodeoxyu...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1972-01, Vol.69 (1), p.78-82
Main Authors: Hampar, Berge, Derge, Jeffery G., Martos, Lidia M., Walker, John L.
Format: Article
Language:English
Subjects:
Animals
Antigens, Viral
Biological Sciences: Microbiology
Bromodeoxyuridine - pharmacology
Cell Line
Cell lines
Cell nucleus
Cell Transformation, Neoplastic
Cellular immunity
Culture Media
DNA, Viral - biosynthesis
Drug Resistance, Microbial
Fluorescent Antibody Technique
Fluorescent antibody techniques
Genomes
Genotype
Herpesviridae - metabolism
Human herpesvirus 4
Humans
Immune Sera
In Vitro Techniques
Lymphocytes - drug effects
Lymphocytes - enzymology
Lymphocytes - metabolism
Microscopy, Electron
Rabbits
T lymphocytes
Thymidine Kinase - metabolism
Thymine Nucleotides - metabolism
Viral antigens
Virions
Virus Replication - drug effects
Viruses
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Summary:The Raji line of human lymphoblastoid cells, which does not show expression of Epstein-Barr virus, was made resistant to 5-bromodeoxyuridine. Within several weeks after removal of the drug, Epstein-Barr virus particles were detected in the cells. The sequence of virus appearance in the 5-bromodeoxyuridine-resistant cells indicated drug-induced activation of a repressed Epstein-Barr virus genome.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.69.1.78