Embryonic trafficking of γδ T cells to skin is dependent on E/P selectin ligands and CCR4

Dendritic epidermal T cells (DETC) express an invariant Vγ3/Vδ1 T-cell receptor, appear in fetal epidermis, and form a population of resident epidermal T cells. Their temporal development in the thymus has been studied extensively. However, little is known about the mechanisms involved in the embryo...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2010-04, Vol.107 (16), p.7443-7448
Main Authors: Jiang, Xiaodong, Campbell, James J, Kupper, Thomas S
Format: Article
Language:English
Subjects:
Biological Sciences
Epidermal cells
Epidermis
Homing
Ligands
Mice
Selectins
Skin
T cell antigen receptors
T lymphocytes
T lymphoid precursor cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Dendritic epidermal T cells (DETC) express an invariant Vγ3/Vδ1 T-cell receptor, appear in fetal epidermis, and form a population of resident epidermal T cells. Their temporal development in the thymus has been studied extensively. However, little is known about the mechanisms involved in the embryonic trafficking of DETC from thymus to epidermis. We demonstrate that DETC in adult skin, as well as the DETC precursors in fetal thymus, express E and P selectin ligands (E- and P-lig). Mice deficient in α1,3 fucosyltransferases IV and VII (FTIV/VII) cannot synthesize the carbohydrate motifs that form key elements of these selectin ligands. The numbers of DETC in the epidermis of FTIV/VII⁻/⁻ mice were dramatically reduced compared with normal mice. However, the development of DETC precursors in fetal thymus was identical in normal and FTIV/VII⁻/⁻ mice, suggesting that the DETC precursors produced in FTIV/VII⁻/⁻ mice could not traffic effectively to skin because they lack E- and P-lig. We tested this hypothesis by daily injection of blocking antibodies against E and P selectin into pregnant mice. Mice born from dams treated with anti-selectin antibodies, but not those born from dams treated with isotype control, had significantly diminished numbers of DETC. To test the role of chemokine receptors in DETC skin homing, we examined skin from CCR4⁻/⁻ and CCR10⁻/⁻ mice, respectively. DETC were significantly reduced in CCR4⁻/⁻ mice but were present at normal levels in CCR10⁻/⁻ mice. Our results present evidence for the crucial role of trafficking molecules in embryonic migration of DETC precursors to skin.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0912943107