Drosophila Host Defense after Oral Infection by an Entomopathogenic Pseudomonas Species

Drosophila has been shown to be a valuable model for the investigation of host-pathogen interactions. Study of the Drosophila immune response has been hampered, however, by the lack of true Drosophila pathogens. In nearly all studies reported, the bacteria used were directly injected within the body...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2005-08, Vol.102 (32), p.11414-11419
Main Authors: Vodovar, Nicolas, Vinals, Marisa, Liehl, Peter, Basset, Alan, Degrouard, Jeril, Spellman, Paul, Boccard, Frédéric, Lemaitre, Bruno, Anderson, Kathryn V.
Format: Article
Language:English
Subjects:
Animals
Antimicrobial Cationic Peptides - genetics
Antimicrobial Cationic Peptides - metabolism
Antimicrobials
Bacteria
Base Sequence
Biological Sciences
Digestive System - metabolism
Digestive System - ultrastructure
Drosophila
Drosophila - immunology
Drosophila - metabolism
Drosophila - microbiology
Gene expression
Gene Expression Profiling
Gene Expression Regulation - immunology
Genes
Genes, Bacterial - genetics
Guadeloupe
Immune response
Immune system
Infections
Insect larvae
Insects
Larva - immunology
Larva - metabolism
Larva - microbiology
Larvae
Larval development
Microarray Analysis
Microscopy, Electron
Molecular Sequence Data
Pathogens
Phylogeny
Pseudomonas
Pseudomonas - genetics
Pseudomonas - immunology
Pseudomonas - pathogenicity
Sequence Analysis, DNA
Species Specificity
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Summary:Drosophila has been shown to be a valuable model for the investigation of host-pathogen interactions. Study of the Drosophila immune response has been hampered, however, by the lack of true Drosophila pathogens. In nearly all studies reported, the bacteria used were directly injected within the body cavity of the insect, bypassing the initial steps of a natural interaction. Here, we report the identification of a previously uncharacterized bacterial species, Pseudomonas entomophila (Pe), which has the capacity to induce the systemic expression of antimicrobial peptide genes in Drosophila after ingestion. In contrast to previously identified bacteria, Pe is highly pathogenic to both Drosophila larvae and adults, and its persistence in larvae leads to a massive destruction of gut cells. Using this strain, we have analyzed the modulation of the larval transcriptome upon bacterial infection. We found that natural infection by Pe induces a dramatic change in larval gene expression. In addition to immunity genes, our study identifies many genes associated with Pe pathogenesis that have been previously unreported.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0502240102