Paracrine regulation of neural crest EMT by placodal MMP28

Epithelial–mesenchymal transition (EMT) is an early event in cell dissemination from epithelial tissues. EMT endows cells with migratory, and sometimes invasive, capabilities and is thus a key process in embryo morphogenesis and cancer progression. So far, matrix metalloproteinases (MMPs) have not b...

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Bibliographic Details
Published in:PLoS biology 2023-08, Vol.21 (8), p.e3002261-e3002261
Main Authors: Gouignard, Nadège, Bibonne, Anne, Mata, João F., Bajanca, Fernanda, Berki, Bianka, Barriga, Elias H., Saint-Jeannet, Jean-Pierre, Theveneau, Eric
Format: Article
Language:English
Subjects:
Apoptosis
Biology and Life Sciences
Cancer
Cell adhesion & migration
Cell migration
Computer and Information Sciences
Embryos
Extracellular matrix
Fibrosis
Genes
Hybridization
Matrix metalloproteinase
Matrix metalloproteinases
Medical prognosis
Morphogenesis
Neural crest
Paracrine signalling
Research and Analysis Methods
Wound healing
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Summary:Epithelial–mesenchymal transition (EMT) is an early event in cell dissemination from epithelial tissues. EMT endows cells with migratory, and sometimes invasive, capabilities and is thus a key process in embryo morphogenesis and cancer progression. So far, matrix metalloproteinases (MMPs) have not been considered as key players in EMT but rather studied for their role in matrix remodelling in later events such as cell migration per se. Here, we used Xenopus neural crest cells to assess the role of MMP28 in EMT and migration in vivo. We show that a catalytically active MMP28, expressed by neighbouring placodal cells, is required for neural crest EMT and cell migration. We provide strong evidence indicating that MMP28 is imported in the nucleus of neural crest cells where it is required for normal Twist expression. Our data demonstrate that MMP28 can act as an upstream regulator of EMT in vivo raising the possibility that other MMPs might have similar early roles in various EMT-related contexts such as cancer, fibrosis, and wound healing.
ISSN:1545-7885
1544-9173
1545-7885
DOI:10.1371/journal.pbio.3002261