Genetic analysis of the rice jasmonate receptors reveals specialized functions for OsCOI2

COI1-mediated perception of jasmonate is critical for plant development and responses to environmental stresses. Monocots such as rice have two groups of COI genes due to gene duplication: OsCOI1a and OsCOI1b that are functionally equivalent to the dicotyledons COI1 and OsCOI2 whose function remains...

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Published in:PloS one 2023-09, Vol.18 (9), p.e0291385
Main Authors: Nguyen, Hieu Trang, Cheaib, Mohamad, Fournel, Marie, Rios, Maelle, Gantet, Pascal, Laplaze, Laurent, Guyomarc'h, Soazig, Riemann, Michael, Heitz, Thierry, Petitot, Anne-Sophie, Champion, Antony
Format: Article
Language:English
Subjects:
Amino acids
Analysis
Biochemistry, Molecular Biology
Biology and Life Sciences
Computer and Information Sciences
CRISPR
Development Biology
Environmental stress
Fertility
Gene duplication
Gene expression
Genes
Genetic analysis
Genetic aspects
Genetic research
Genetics
Genomics
Humidity
Jasmonates
Jasmonic acid
Life Sciences
Mutation
Phenotypes
Physiology
Plants (botany)
Plants genetics
Proteins
Receptors
Redundancy
Research and Analysis Methods
Rice
Seeds
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Summary:COI1-mediated perception of jasmonate is critical for plant development and responses to environmental stresses. Monocots such as rice have two groups of COI genes due to gene duplication: OsCOI1a and OsCOI1b that are functionally equivalent to the dicotyledons COI1 and OsCOI2 whose function remains unclear. In order to assess the function of OsCOI2 and its functional redundancy with COI1 genes, we developed a series of rice mutants in the 3 genes OsCOI1a, OsCOI1b and OsCOI2 by CRISPR Cas9-mediated editing and characterized their phenotype and responses to jasmonate. Characterization of OsCOI2 uncovered its important roles in root, leaf and flower development. In particular, we show that crown root growth inhibition by jasmonate relies on OsCOI2 and not on OsCOI1a nor on OsCOI1b, revealing a major function for the non-canonical OsCOI2 in jasmonate-dependent control of rice root growth. Collectively, these results point to a specialized function of OsCOI2 in the regulation of plant development in rice and indicate that sub-functionalisation of jasmonate receptors has occurred in the monocot phylum.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0291385