Multidrug resistant Klebsiella Pneumoniae reservoir and their capsular resistance genes in cow farms of district Peshawar, Pakistan

Klebsiella pneumoniae is a major zoonotic pathogen that causes a variety of severe illnesses as well as mastitis. The distribution of mastitis-causing K. Pneumoniae and its virulence factors vary by country and geographical location. The present study aimed to find out the occurrence of Multidrug-re...

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Bibliographic Details
Published in:PloS one 2023-02, Vol.18 (2), p.e0282245-e0282245
Main Authors: Saddam, Saddam, Khan, Muddasir, Jamal, Muhsin, Rehman, Sadeeq Ur, Slama, Petr, Horky, Pavel
Format: Article
Language:English
Subjects:
Analysis
Animals
Animals, Domestic
Antibiotics
Antigens
Bacteria
Bacterial pneumonia
Biology and Life Sciences
Care and treatment
Cattle
Causes of
Ceftazidime
Chi-square test
Dairy cattle
Dairy farming
Dairy farms
Dairy industry
Dendritic cells
Diagnosis
Diseases
Drug resistance
Drug resistance in microorganisms
Farms
Female
Genes
Genetic aspects
Genetic research
Geographical distribution
Geographical locations
Growth
High resistance
Humans
Hygiene
Imipenem
Immune system
K1 gene
Klebsiella
Klebsiella infections
Klebsiella pneumoniae
Klebsiella pneumoniae - genetics
Laboratories
Livestock
Livestock industry
Mastitis
Medicine and Health Sciences
Milk
Multidrug resistance
Multidrug resistant organisms
Pakistan - epidemiology
Pathogenesis
Pathogens
People and Places
Peptides
Pneumonia
Prevention
Serotypes
Statistical analysis
Tetracycline
Tetracyclines
Vancomycin
Virulence
Virulence factors
Zoonoses
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Summary:Klebsiella pneumoniae is a major zoonotic pathogen that causes a variety of severe illnesses as well as mastitis. The distribution of mastitis-causing K. Pneumoniae and its virulence factors vary by country and geographical location. The present study aimed to find out the occurrence of Multidrug-resistant (MDR) K. Pneumoniae and their capsular resistance genes which were undocumented previously in cow farms of district Peshawar, Pakistan. A total of 700 milk samples from symptomatic mastitic cows were screened for MDR K. Pneumoniae. Furthermore, the characterization of capsular resistance genes was done by molecular techniques. Among these samples, K. pneumoniae was found 180/700 (25.7%), while MDR K. pneumoniae was found 80/180 (44.4%). The antibiogram analysis revealed high resistance to Vancomycin (95%) while highly sensitive to Ceftazidime (80%). The distribution of capsular genes shows the most common serotype K2 gene 39/80 (48.7%), followed by serotype K1 gene 34/80 (42.5%), serotype K5 17/80 (21.2%), and serotype K54 13/80 (16.2), respectively. Moreover, the co-occurrence of serotypes K1+K2 was found at 11.25%, KI+K5 was 05%, K1+K54 was 3.75%, and K2+K5 was 7.5%, respectively. A statistically significant association (p ≤ 0.05) was found between predicted and discovered K. pneumoniae values. In conclusion, the presence of MDR K. pneumoniae in combination with capsular genes may be a possible threat to dairy farm animals and humans in Peshawar, Pakistan. It may give us special attention to follow up on hygienic practices in livestock management.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0282245