A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins

The western corn rootworm (WCR) Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) remains one of the economically most important pests of maize (Zea mays) due to its adaptive capabilities to pest management options. This includes the ability to develop resistance to some of the commercial p...

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Bibliographic Details
Published in:PloS one 2023-02, Vol.18 (2), p.e0267220
Main Authors: Jabeur, Rania, Guyon, Virginie, Toth, Szabolcs, Pereira, Adriano E, Huynh, Man P, Selmani, Zakia, Boland, Erin, Bosio, Mickael, Beuf, Laurent, Clark, Pete, Vallenet, David, Achouak, Wafa, Audiffrin, Carine, Torney, François, Paul, Wyatt, Heulin, Thierry, Hibbard, Bruce E, Toepfer, Stefan, Sallaud, Christophe
Format: Article
Language:English
Subjects:
Agricultural production
Amino acids
Analysis
Animals
Bacillus thuringiensis - genetics
Bacteria
Bacterial Proteins - metabolism
Binding sites
Bioassays
Biology and Life Sciences
Chryseobacterium
Chryseobacterium - metabolism
Coleoptera - genetics
Corn
Diabrotica virgifera virgifera
Diseases and pests
Endotoxins - metabolism
Environmental aspects
Genetic aspects
Genomes
Growth
Health aspects
Hypotheses
Insecticide Resistance
Insecticides
Larva - metabolism
Larvae
Life Sciences
Medicine and Health Sciences
Mode of action
Peptides
Pest control
Pest Control, Biological
Pesticides
Pesticides - pharmacology
Pests
Plants, Genetically Modified - metabolism
Proteins
Recombinant proteins
Research and Analysis Methods
Vegetal Biology
Zea mays - genetics
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Summary:The western corn rootworm (WCR) Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) remains one of the economically most important pests of maize (Zea mays) due to its adaptive capabilities to pest management options. This includes the ability to develop resistance to some of the commercial pesticidal proteins originating from different strains of Bacillus thuringiensis. Although urgently needed, the discovery of new, environmentally safe agents with new modes of action is a challenge. In this study we report the discovery of a new family of binary pesticidal proteins isolated from several Chryseobacterium species. These novel binary proteins, referred to as GDI0005A and GDI0006A, produced as recombinant proteins, prevent growth and increase mortality of WCR larvae, as does the bacteria. These effects were found both in susceptible and resistant WCR colonies to Cry3Bb1 and Cry34Ab1/Cry35Ab1 (reassigned Gpp34Ab1/Tpp35Ab1). This suggests GDI0005A and GDI0006A may not share the same binding sites as those commercially deployed proteins and thereby possess a new mode of action. This paves the way towards the development of novel biological or biotechnological management solutions urgently needed against rootworms.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0267220