Effect of silver nanoparticles associated with fluoride on the progression of root dentin caries in vitro

To assess the anti-proteolytic effect and potential to inhibit dentin root caries progression of a silver nanoparticle and fluoride solution (CNanoF) in comparison to silver diamine fluoride (SDF). 48 specimens of root dentin artificial caries lesion were treated with 38% SDF, CNanoF, CNano or F (n...

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Published in:PloS one 2023-01, Vol.18 (1), p.e0277275
Main Authors: Dias, Flaviana Alves, Vidal, Cristina M P, Comnick, Carissa L, Xie, Xian Jin, Berger, Sandrine Bittencourt
Format: Article
Language:English
Subjects:
Analysis
Biology and Life Sciences
Care and treatment
Cariostatic Agents
Collagen
Collagen - pharmacology
Collagenase
Collagenases - pharmacology
Collagens
Confocal microscopy
Cycles
Degradation
Demineralization
Demineralizing
Dental caries
Dental Caries - drug therapy
Dental Caries - pathology
Dental Caries - prevention & control
Dental Caries Susceptibility
Dentin
Development and progression
Diamines
Enamel
Engineering and Technology
Fluoride
Fluoride treatments
Fluorides
Fluorides, Topical - pharmacology
Gelatinase A
Health aspects
Humans
Hydroxyproline
Integrity
Kruskal-Wallis test
Lesions
Medicine and Health Sciences
Metal Nanoparticles
Methods
Microhardness
Nanoparticles
Older people
Permeability
Physical Sciences
Proteolysis
Quaternary Ammonium Compounds - pharmacology
Remineralization
Research and Analysis Methods
Root Caries - drug therapy
Root Caries - pathology
Scanning microscopy
Silicon carbide
Silver
Silver - pharmacology
Silver Compounds - pharmacology
Statistical analysis
Teeth
Variance analysis
Weight loss
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Summary:To assess the anti-proteolytic effect and potential to inhibit dentin root caries progression of a silver nanoparticle and fluoride solution (CNanoF) in comparison to silver diamine fluoride (SDF). 48 specimens of root dentin artificial caries lesion were treated with 38% SDF, CNanoF, CNano or F (n = 6 per group). Ph cycling with demineralization and remineralization solutions simulated caries lesion progression. In addition, specimens were incubated with or without bacterial collagenase in the remineralization solution to induce dentin proteolytic degradation. Dentin degradation was assessed by weight loss rate and hydroxyproline (Hyp) release. Changes in cross-sectional microhardness, and lesion permeability and collagen integrity as determined by confocal laser scanning microscopy indicated potential for further demineralization inhibition. The effect of the solutions on the activity of metalloproteinases (MMP) -2 and -9 was also investigated. Statistical analysis consisted of ANOVA, Kruskal-Wallis, and linear mixed models with post-hoc pairwise Tukey, Dunn, and t-tests (α = 0.05). Treatment with SDF resulted in lower weight loss rate than did other solutions, but all groups showed similar Hyp release (p = 0.183). SDF resulted in greater microhardness at superficial layers of the caries lesions (p0.05), with or without the use of collagenase (p = 0.58). No statistically significant difference was noted among solutions regarding collagen integrity after pH cycling; however, SDF-treated dentin had a significant decrease in collagen integrity when collagenase was used (p = 0.003). Interestingly, only SDF was able to completely inactivate MMP-2 and -9. CNanoF and SDF both potentially prevent dentin degradation during caries lesion progression in vitro; however, SDF was more effective at inhibiting further tissue demineralization.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0277275