A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum

The genetic and molecular basis of flagellar motility has been investigated for several decades, with innovative research strategies propelling advances at a steady pace. Furthermore, as the phenomenon is examined in diverse bacteria, new taxon-specific regulatory and structural features are being e...

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Published in:PloS one 2023-01, Vol.18 (1), p.e0279936-e0279936
Main Authors: Calvopina-Chavez, Diana G, Howarth, Robyn E, Memmott, Audrey K, Pech Gonzalez, Oscar H, Hafen, Caleb B, Jensen, Kyson T, Benedict, Alex B, Altman, Jessica D, Burnside, Brittany S, Childs, Justin S, Dallon, Samuel W, DeMarco, Alexa C, Flindt, Kirsten C, Grover, Sarah A, Heninger, Elizabeth, Iverson, Christina S, Johnson, Abigail K, Lopez, Jack B, Meinzer, McKay A, Moulder, Brook A, Moulton, Rebecca I, Russell, Hyrum S, Scott, Tiana M, Shiobara, Yuka, Taylor, Mason D, Tippets, Kathryn E, Vainerere, Kayla M, Von Wallwitz, Isabella C, Wagley, Madison, Wiley, Megumi S, Young, Naomi J, Griffitts, Joel S
Format: Article
Language:English
Subjects:
Agrobacterium
Agrobacterium - genetics
Antibiotics
Bacteria
Bacterial Proteins - genetics
Biology and Life Sciences
Biosynthesis
Control
Dosage and administration
E coli
Ethylenediaminetetraacetic acid
Flagella
Flagella (Microbiology)
Flagella - metabolism
Gene expression
Gene Expression Regulation, Bacterial
Genes
Genetic screening
Glycerol
Identification and classification
Innovations
Medicine and Health Sciences
Methods
Molecular genetics
Motility
Mutagenesis
Neomycin
Phenotypes
Physiological aspects
Proteins
Research and analysis methods
Stabbings
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Summary:The genetic and molecular basis of flagellar motility has been investigated for several decades, with innovative research strategies propelling advances at a steady pace. Furthermore, as the phenomenon is examined in diverse bacteria, new taxon-specific regulatory and structural features are being elucidated. Motility is also a straightforward bacterial phenotype that can allow undergraduate researchers to explore the palette of molecular genetic tools available to microbiologists. This study, driven primarily by undergraduate researchers, evaluated hundreds of flagellar motility mutants in the Gram-negative plant-associated bacterium Agrobacterium fabrum. The nearly saturating screen implicates a total of 37 genes in flagellar biosynthesis, including genes of previously unknown function.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0279936