Evaluation of intron-1 of odorant-binding protein-1 of Anopheles stephensi as a marker for the identification of biological forms or putative sibling species

Anopheles stephensi, an invasive malaria vector, has been reported to have three biological forms identifiable mainly based on the number of ridges present on the egg's floats. Recently, the first intron of the odorant-binding protein-1 (AsteObp1) has been introduced as a molecular marker for t...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2022-07, Vol.17 (7), p.e0270760-e0270760
Main Authors: Singh, Om P, Mishra, Shobhna, Sharma, Gunjan, Sindhania, Ankita, Kaur, Taranjeet, Sreehari, U, Das, Manoj K, Kapoor, Neera, Gupta, Bhavna
Format: Article
Language:English
Subjects:
Analysis
Anopheles
Anopheles stephensi
Biology and Life Sciences
Biomarkers
Cloning
Cloning vectors
Deoxyribonucleic acid
DNA
DNA sequencing
Eggs
Experiments
Floats
Gene polymorphism
Gene sequencing
Genetic aspects
Genetic vectors
Haplotypes
Heterozygosity
Identification
Inbreeding
Laboratories
Malaria
Markers
Medicine and Health Sciences
Mosquitoes
Nucleotides
Odorant-binding protein
Polymorphism
Populations
Proteins
Research and Analysis Methods
Ridges
Sibling species
Urban areas
Vector-borne diseases
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Anopheles stephensi, an invasive malaria vector, has been reported to have three biological forms identifiable mainly based on the number of ridges present on the egg's floats. Recently, the first intron of the odorant-binding protein-1 (AsteObp1) has been introduced as a molecular marker for the identification of these forms, and based on this marker, the presence of three putative sibling species (designated as species A, B and C) has been proposed. However, there is no data on the association of proposed markers with biological form or putative species on field populations. Field collected and laboratory-reared An. stephensi were characterized for biological forms based on the number of ridges on the egg's float. DNA sequencing of the partial AsteObp1 gene of An. stephensi individuals were performed by Sanger's method, either directly or after cloning with a plasmid vector. Additionally, AsteObp1 sequences of various laboratory lines of An. stephensi were retrieved from a public sequence database. AsteObp1 intron-1 in Indian An. stephensi populations are highly polymorphic with the presence of more than 13 haplotypes exhibiting nucleotides as well as length-polymorphism (90-to-121 bp). No specific haplotype or a group of closely related haplotypes of intron-1 was found associated with any biological form identified morphologically. High heterozygosity for this marker with a low inbreeding coefficient in field and laboratory populations indicates that this marker is not suitable for the delimitation of putative sibling species, at least in Indian populations. AsteObp1 cannot serve as a marker for identifying biological forms of An. stephensi or putative sibling species in Indian populations.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0270760