Association between aqueous humor cytokines and postoperative corneal endothelial cell loss after Descemet stripping automated endothelial keratoplasty

This study measured the intraoperative anterior aqueous humor concentrations of various cytokines during corneal endothelial transplantation and searched for relationships between these concentrations and postoperative corneal endothelial cell (CEC) depletion. We recruited 30 consecutive patients wh...

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Bibliographic Details
Published in:PloS one 2021-12, Vol.16 (12), p.e0260963-e0260963
Main Authors: Okabe, Tatsu, Kobayashi, Wataru, Hariya, Takehiro, Yokokura, Shunji, Nakazawa, Toru
Format: Article
Language:English
Subjects:
Aged
Analysis
Aqueous humor
Aqueous Humor - metabolism
Aqueous humour
Automation
Biology and Life Sciences
Cornea
Corneal Diseases - surgery
Corneal Endothelial Cell Loss - etiology
Corneal Endothelial Cell Loss - metabolism
Corneal Endothelial Cell Loss - pathology
Corneal transplantation
Correlation coefficient
Correlation coefficients
Cytokines
Cytokines - metabolism
Depletion
Descemet Stripping Endothelial Keratoplasty - adverse effects
Disease control
Dosage and administration
Endothelial cells
Endothelium, Corneal - cytology
Endothelium, Corneal - transplantation
Eye surgery
Female
Glaucoma
Granulocytes
Growth factors
Hospitals
Humans
IL-1β
Immunoassay
Inflammation
Interleukin 1
Interleukin 7
IP-10 protein
Iris
Lasers
Lymphocytes
Macrophage inflammatory protein
Macrophage inflammatory protein 1
Male
Medicine
Medicine and Health Sciences
Middle Aged
Multiple regression analysis
Ophthalmic solutions
Ophthalmology
Postoperative Complications - etiology
Postoperative Complications - metabolism
Postoperative Complications - pathology
Prognosis
Proteins
RANTES
Regression analysis
Retrospective Studies
Statistical analysis
Surgery
Surgical techniques
Transplantation
University graduates
Citations: Items that this one cites
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Summary:This study measured the intraoperative anterior aqueous humor concentrations of various cytokines during corneal endothelial transplantation and searched for relationships between these concentrations and postoperative corneal endothelial cell (CEC) depletion. We recruited 30 consecutive patients who underwent corneal endothelial transplantation with Descemet's stripping automated endothelial keratoplasty (DSAEK) at Tohoku University Hospital between February 2014 and July 2017. During surgery, we obtained aqueous humor samples and later measured the concentrations of 27 cytokines with a Multiplex Bead Assay (Bio-Plex Pro). We counted CECs 1, 6 and 12 months after surgery, and used Spearman's rank correlation coefficient to identify relationships between CEC depletion and the concentrations of detected cytokines. The loss of CECs 1-6 months after surgery was significantly correlated with IL-7, IP-10, MIP-1a and MIP-1b concentrations (-0.67, -0.48, -0.39, and -0.45, respectively, all P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0260963