The first report of porcine parvovirus 7 (PPV7) in Colombia demonstrates the presence of variants associated with modifications at the level of the VP2-capsid protein

There are a wide variety of porcine parvoviruses (PPVs) referred to as PPV1 to PPV7. The latter was discovered in 2016 and later reported in some countries in America, Asia, and Europe. PPV7 as a pathogenic agent or coinfection with other pathogens causing disease has not yet been determined. In the...

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Bibliographic Details
Published in:PloS one 2021-12, Vol.16 (12), p.e0258311-e0258311
Main Authors: Vargas-Bermudez, Diana S, Rendon-Marin, Santiago, Ruiz-Saenz, Julian, Mogollón, Dario, Jaime, Jairo
Format: Article
Language:English
Subjects:
Amino acids
Analysis
Animals
Antigens, Viral - chemistry
Antigens, Viral - genetics
Biology and Life Sciences
Capsid protein
Capsid Proteins - chemistry
Capsid Proteins - genetics
Care and treatment
Colombia
Computer and Information Sciences
Diagnosis
Epitopes
Fetuses
Genes
Genomes
Hogs
Homology
Immune response
Insertion
Maximum likelihood method
Nucleotides
Parvoviridae Infections - genetics
Parvoviridae Infections - veterinary
Parvovirus infections
Parvovirus, Porcine - chemistry
Parvovirus, Porcine - genetics
Parvovirus, Porcine - isolation & purification
Parvoviruses
Phylogenetics
Phylogeny
Protein Domains
Protein structure
Proteins
Research and Analysis Methods
Risk factors
Secondary structure
Strains (organisms)
Swine
Swine Diseases - genetics
Swine Diseases - virology
Viruses
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Summary:There are a wide variety of porcine parvoviruses (PPVs) referred to as PPV1 to PPV7. The latter was discovered in 2016 and later reported in some countries in America, Asia, and Europe. PPV7 as a pathogenic agent or coinfection with other pathogens causing disease has not yet been determined. In the present study, we report the identification of PPV7 for the first time in Colombia, where it was found retrospectively since 2015 in 40% of the provinces that make up the country (13/32), and the virus was ratified for 2018 in 4/5 provinces evaluated. Additionally, partial sequencing (nucleotides 380 to 4000) was performed of four Colombian strains completely covering the VP2 and NS1 viral genes. A sequence identity greater than 99% was found when comparing them with reference strains from the USA and China. In three of the four Colombian strains, an insertion of 15 nucleotides (five amino acids) was found in the PPV7-VP2 capsid protein (540-5554 nt; 180-184 aa). Based on this insertion, the VP2 phylogenetic analysis exhibited two well-differentiated evolutionarily related groups. To evaluate the impact of this insertion on the structure of the PPV7-VP2 capsid protein, the secondary structure of two different Colombian strains was predicted, and it was determined that the insertion is located in the coil region and not involved in significant changes in the structure of the protein. The 3D structure of the PPV7-VP2 capsid protein was determined by threading and homology modeling, and it was shown that the insertion did not imply a change in the shape of the protein. Additionally, it was determined that the insertion is not involved in suppressing a potential B cell epitope, although the increase in length of the epitope could affect the interaction with molecules that allow a specific immune response.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0258311