Proliferation, apoptosis and their regulatory protein expression in colorectal adenomas and serrated lesions

Adenomas and serrated lesions represent heterogeneous sets of early precursors in the colorectum with varying malignant potential. They are often distinguished by their histopathologic differences, but little is known about potential differences in regulation of epithelial proliferation and apoptosi...

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Published in:	PloS one 2021-11, Vol.16 (11), p.e0258878-e0258878
Main Authors:	Figueiredo, Jane C, Passarelli, Michael N, Wei, Wei, Ahnen, Dennis J, Morris, Jeffrey S, Corley, Lynda, Mehta, Trupti, Bartley, Angela N, McKeown-Eyssen, Gail, Bresalier, Robert S, Barry, Elizabeth L, Goel, Ajay, Hernandez Mesa, Goretti, Hamilton, Stanley R, Baron, John A
Format:	Article
Language:	eng
Subjects:	Adenoma - pathology Aged Apoptosis Bax protein Biology and Life Sciences Blood proteins Cancer Caspase-3 Cell Proliferation Colonoscopy Colorectal cancer Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology Cyclin D1 Cyclin-dependent kinase inhibitor p21 Epidemiology Female Gastroenterology Genetic aspects Health aspects Hepatology Humans Laboratories Lesions Male Medicine Medicine and Health Sciences Middle Aged Neoplasm Proteins - metabolism Pathology Polyps Protein expression Proteins Research and Analysis Methods Survivin Tissue analysis Tumors
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Summary:	Adenomas and serrated lesions represent heterogeneous sets of early precursors in the colorectum with varying malignant potential. They are often distinguished by their histopathologic differences, but little is known about potential differences in regulation of epithelial proliferation and apoptosis. We conducted a protein expression analysis using tissue microarrays of 625 colorectal adenomas and 142 serrated lesions to determine potential differences in regulation of epithelial proliferation and apoptosis. We quantitated proliferation with Ki-67; apoptosis with activated caspase-3 (CASP3); up- and down-regulators of proliferation with cyclin D1, p16INK2, and p21Cip1; and apoptosis regulators with BAX, BCL2, and survivin. Linear mixed effects models and circos diagrams were used to determine relationships among expression and lesion characteristics. Adenomas had a significantly higher CASP-3 labeling index (LI) than serrated lesions, resulting in a lower net growth ratio (Ki-67 LI/activated CASP-3 LI, p-value
ISSN:	1932-6203 1932-6203