From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners' patients

To evaluate the pathogenicity of a broad range of 11 possible gastroenteritis viruses, by means of statistical relationships with cases vs. controls, or Ct-values, in order to establish the most appropriate diagnostic panel for our general practitioner (GP) patients in the Netherlands (2010-2012). A...

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Bibliographic Details
Published in:	PloS one 2021-11, Vol.16 (11), p.e0258680-e0258680
Main Authors:	Bruijnesteijn van Coppenraet, Lesla E S, Flipse, Jacky, Wallinga, Janny A, Vermeer, Marloes, van der Reijden, Wil A, Weel, Jan F L, van der Zanden, Adri G M, Schuurs, Theo A, Ruijs, Gijs J H M
Format:	Article
Language:	eng
Subjects:	Adenoviridae - genetics Adenoviridae - isolation & purification Adenoviridae - pathogenicity Adenoviruses Biology and life sciences Bocavirus - genetics Bocavirus - isolation & purification Bocavirus - pathogenicity Child, Preschool Confidence intervals Diagnosis Diagnostic systems Diarrhea Enterovirus Infections - diagnosis Enterovirus Infections - genetics Enterovirus Infections - pathology Enterovirus Infections - virology Enteroviruses Epidemiology Evaluation Feces - virology Female Gastroenteritis Gastroenteritis - diagnosis Gastroenteritis - genetics Gastroenteritis - pathology Gastroenteritis - virology General Practitioners Health care Humans Infant Infectious diseases Laboratories Male Medical screening Medicine and Health Sciences Methods Norovirus - genetics Norovirus - isolation & purification Norovirus - pathogenicity Pathogenicity Pathogens Patients Physicians (General practice) Population Public health Rotavirus Rotavirus - genetics Rotavirus - isolation & purification Rotavirus - pathogenicity Sapovirus - genetics Sapovirus - isolation & purification Sapovirus - pathogenicity Services Statistical analysis Statistical methods Viruses
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Summary:	To evaluate the pathogenicity of a broad range of 11 possible gastroenteritis viruses, by means of statistical relationships with cases vs. controls, or Ct-values, in order to establish the most appropriate diagnostic panel for our general practitioner (GP) patients in the Netherlands (2010-2012). Archived stool samples from 1340 cases and 1100 controls were retested using internally controlled multiplex real-time PCRs for putative pathogenic gastroenteritis viruses: adenovirus, astrovirus, bocavirus, enterovirus, norovirus GI and GII, human parechovirus, rotavirus, salivirus, sapovirus, and torovirus. The prevalence of any virus in symptomatic cases and asymptomatic controls was 16.6% (223/1340) and 10.2% (112/1100), respectively. Prevalence of astrovirus (adjusted odds ratio (aOR) 10.37; 95% confidence interval (CI) 1.34-80.06) and norovirus GII (aOR 3.10; CI 1.62-5.92) was significantly higher in cases versus controls. Rotavirus was encountered only in cases. We did not find torovirus and there was no statistically significant relationship with cases for salivirus (aOR 1,67; (CI) 0.43-6.54)), adenovirus non-group F (aOR 1.20; CI 0.75-1.91), bocavirus (aOR 0.85; CI 0.05-13.64), enterovirus (aOR 0.83; CI 0.50-1.37), human parechovirus (aOR 1.61; CI 0.54-4.77) and sapovirus (aOR 1.15; CI 0.67-1.98). Though adenovirus group F (aOR 6.37; CI 0.80-50.92) and norovirus GI (aOR 2.22, CI: 0.79-6.23) are known enteropathogenic viruses and were more prevalent in cases than in controls, this did not reach significance in this study. The Ct value did not discriminate between carriage and disease in PCR-positive subjects. In our population, diagnostic gastroenteritis tests should screen for adenovirus group F, astrovirus, noroviruses GI and GII, and rotavirus. Case-control studies as ours are lacking and should also be carried out in populations from other epidemiological backgrounds.
ISSN:	1932-6203 1932-6203