Identification of cell lines CL-14, CL-40 and CAL-51 as suitable models for SARS-CoV-2 infection studies

The SARS-CoV-2 pandemic is a major global threat that sparked global research efforts. Pre-clinical and biochemical SARS-CoV-2 studies firstly rely on cell culture experiments where the importance of choosing an appropriate cell culture model is often underestimated. We here present a bottom-up appr...

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Published in:PloS one 2021-08, Vol.16 (8), p.e0255622-e0255622
Main Authors: Pommerenke, Claudia, Rand, Ulfert, Uphoff, Cord C, Nagel, Stefan, Zaborski, Margarete, Hauer, Vivien, Kaufmann, Maren, Meyer, Corinna, Denkmann, Sabine A, Riese, Peggy, Eschke, Kathrin, Kim, Yeonsu, Safranko, Zeljka Macak, Kurolt, Ivan-Christian, Markotic, Alemka, Cicin-Sain, Luka, Steenpass, Laura
Format: Article
Language:English
Subjects:
ACE2
Angiotensin-converting enzyme 2
Apoptosis
Asymptomatic
Biology and life sciences
Breast cancer
Cancer
Cell culture
Cell lines
Colon
Colon cancer
COVID-19
Datasets
Drug screening
Encyclopedias
Gene expression
Genetic aspects
Hospitals
Identification and classification
Infections
Infectious diseases
Influenza
Medical research
Medicine and health sciences
Medicine, Experimental
Methods
Microorganisms
mRNA
Pandemics
Proteins
Receptors
Research and Analysis Methods
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Supervision
Tumor cell lines
Viral diseases
Viruses
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Summary:The SARS-CoV-2 pandemic is a major global threat that sparked global research efforts. Pre-clinical and biochemical SARS-CoV-2 studies firstly rely on cell culture experiments where the importance of choosing an appropriate cell culture model is often underestimated. We here present a bottom-up approach to identify suitable permissive cancer cell lines for drug screening and virus research. Human cancer cell lines were screened for the SARS-CoV-2 cellular entry factors ACE2 and TMPRSS2 based on RNA-seq data of the Cancer Cell Line Encyclopedia (CCLE). However, experimentally testing permissiveness towards SARS-CoV-2 infection, we found limited correlation between receptor expression and permissiveness. This underlines that permissiveness of cells towards viral infection is determined not only by the presence of entry receptors but is defined by the availability of cellular resources, intrinsic immunity, and apoptosis. Aside from established cell culture infection models CACO-2 and CALU-3, three highly permissive human cell lines, colon cancer cell lines CL-14 and CL-40 and the breast cancer cell line CAL-51 and several low permissive cell lines were identified. Cell lines were characterised in more detail offering a broader choice of non-overexpression in vitro infection models to the scientific community. For some cell lines a truncated ACE2 mRNA and missense variants in TMPRSS2 might hint at disturbed host susceptibility towards viral entry.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0255622