Mitochondrial genome of Geomydoecus aurei, a pocket-gopher louse

Parasitic lice demonstrate an unusual array of mitochondrial genome architectures and gene arrangements. We characterized the mitochondrial genome of Geomydoecus aurei, a chewing louse (Phthiraptera: Trichodectidae) found on pocket gophers (Rodentia: Geomyidae) using reads from both Illumina and Oxf...

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Bibliographic Details
Published in:PloS one 2021-07, Vol.16 (7), p.e0254138-e0254138
Main Authors: Spradling, Theresa A, Place, Alexandra C, Campbell, Ashley L, Demastes, James W
Format: Article
Language:English
Subjects:
Analysis
Animals
Base Sequence
Biology
Biology and Life Sciences
Chewing
Chromosomes
Cloning
Conserved sequence
Deoxyribonucleic acid
DNA
DNA biosynthesis
Gene clusters
Gene sequencing
Genes
Genetic aspects
Genetic transcription
Genome, Mitochondrial
Genomes
Geomyces
Geomydoecus aurei
Geomyidae
Gophers - genetics
Lice
Mastication
Medicine and Health Sciences
Mitochondria
Nucleotide sequence
Nucleotides
Open Reading Frames - genetics
Phthiraptera
Population genetics
Protein structure
Replication initiation
Research and Analysis Methods
RNA, Ribosomal - genetics
RNA, Transfer - genetics
rRNA
Secondary structure
Sequence Alignment
Transcription initiation
tRNA
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Summary:Parasitic lice demonstrate an unusual array of mitochondrial genome architectures and gene arrangements. We characterized the mitochondrial genome of Geomydoecus aurei, a chewing louse (Phthiraptera: Trichodectidae) found on pocket gophers (Rodentia: Geomyidae) using reads from both Illumina and Oxford Nanopore sequencing coupled with PCR, cloning, and Sanger sequencing to verify structure and arrangement for each chromosome. The genome consisted of 12 circular mitochondrial chromosomes ranging in size from 1,318 to 2,088 nucleotides (nt). Total genome size was 19,015 nt. All 37 genes typical of metazoans (2 rRNA genes, 22 tRNA genes, and 13 protein-coding genes) were present. An average of 26% of each chromosome was composed of non-gene sequences. Within the non-gene region of each chromosome, there was a 79-nt nucleotide sequence that was identical among chromosomes and a conserved sequence with secondary structure that was always followed by a poly-T region. We hypothesize that these regions may be important in the initiation of transcription and DNA replication, respectively. The G. aurei genome shares 8 derived gene clusters with other chewing lice of mammals, but in G. aurei, genes on several chromosomes are not contiguous.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0254138