Involvement of GLUT1 and GLUT3 in the growth of canine melanoma cells

The rate of glucose uptake dramatically increases in cancer cells even in the presence of oxygen and fully functioning mitochondria. Cancer cells produce ATP by glycolysis rather than oxidative phosphorylation under aerobic conditions, a process termed as the "Warburg effect." In the prese...

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Published in:PloS one 2021-02, Vol.16 (2), p.e0243859-e0243859
Main Authors: Suwabe, Yoko, Nakano, Rei, Namba, Shinichi, Yachiku, Naoya, Kuji, Manami, Sugimura, Mana, Kitanaka, Nanako, Kitanaka, Taku, Konno, Tadayoshi, Sugiya, Hiroshi, Nakayama, Tomohiro
Format: Article
Language:English
Subjects:
Biochemistry
Biology and life sciences
Biomedical materials
Blood vessels
Carbon dioxide
Cell suspensions
Chromosome number
Chromosomes
Cryopreservation
Dehydrogenases
Diseases
Dogs
Drug therapy
Editing
Fluorides
Freezing
Funding
Genetic aspects
Glucose
Glucose metabolism
Health aspects
Incubation
Laboratories
Mammalian cells
Medicine and Health Sciences
Melanoma
Metabolism
Pharmaceuticals
Physical Sciences
Radiation therapy
Reagents
Research and Analysis Methods
Reviews
Skin cancer
Tubes
Water baths
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Summary:The rate of glucose uptake dramatically increases in cancer cells even in the presence of oxygen and fully functioning mitochondria. Cancer cells produce ATP by glycolysis rather than oxidative phosphorylation under aerobic conditions, a process termed as the "Warburg effect." In the present study, we treated canine melanoma cells with the glucose analog 2-deoxy-D-glucose (2-DG) and investigated its effect on cell growth. 2-DG attenuated cell growth in a time- and dose-dependent manner. Cell growth was also inhibited following treatment with the glucose transporter (GLUT) inhibitor WZB-117. The treatment of 2-DG and WZB-117 attenuated the glucose consumption, lactate secretion and glucose uptake of the cells. The mRNA expression of the subtypes of GLUT was examined and GLUT1 and GLUT3 were found to be expressed in melanoma cells. The growth, glucose consumption and lactate secretion of melanoma cells transfected with siRNAs of specific for GLUT1 and GLUT3 was suppressed. These findings suggest that glucose uptake via GLUT1 and GLUT3 plays a crucial role for the growth of canine melanoma cells.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0243859