Adipocyte-induced transdifferentiation of osteoblasts and its potential role in age-related bone loss

Our preliminary findings have lead us to propose bone marrow adipocyte secretions as new contributors to bone loss. Indeed, using a coculture model based on human bone marrow stromal cells, we previously showed that soluble factors secreted by adipocytes induced the conversion of osteoblasts towards...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2021-01, Vol.16 (1), p.e0245014-e0245014
Main Authors: Clabaut, Aline, Grare, Céline, Rolland-Valognes, Gaëlle, Letarouilly, Jean-Guillaume, Bourrier, Chantal, Andersen, Thomas L, Sikjær, Tanja, Rejnmark, Lars, Ejersted, Charlotte, Pastoureau, Philippe, Hardouin, Pierre, Sabatini, Massimo, Broux, Odile
Format: Article
Language:English
Subjects:
Adipocytes
Adipocytes - metabolism
Adipocytes - physiology
Adipose tissue
Age
Aging
Authorship
Biology and Life Sciences
Biomedical materials
Biopsy
Bone loss
Bone marrow
Cell culture
Cell Transdifferentiation - physiology
Cells, Cultured
Clean energy
Coculture Techniques
Development and progression
DNA methylation
DNA-directed RNA polymerase
Endocrinology
Experiments
Fat cells
Forensic science
Gene expression
Gene Expression Profiling
Genetic aspects
Health aspects
Human performance
Humans
Immunohistochemistry
Inflammatory diseases
Internal medicine
Life Sciences
Medicine
Medicine and Health Sciences
Osteoblasts
Osteoblasts - metabolism
Osteoblasts - physiology
Osteoporosis
Osteoporosis - genetics
Osteoporosis - metabolism
Peptidylprolyl isomerase
Physiological aspects
Polymerase chain reaction
Research and Analysis Methods
Ribonucleic acid
Risk factors
RNA
RNA polymerase
RNA polymerase II
Software
Statistical analysis
Transcriptome
Tryptophan
Tryptophan 5-monooxygenase
Tyrosine
Tyrosine 3-monooxygenase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Our preliminary findings have lead us to propose bone marrow adipocyte secretions as new contributors to bone loss. Indeed, using a coculture model based on human bone marrow stromal cells, we previously showed that soluble factors secreted by adipocytes induced the conversion of osteoblasts towards an adipocyte-like phenotype. In this study, microarray gene expression profiling showed profound transcriptomic changes in osteoblasts following coculture and confirmed the enrichment of the adipocyte gene signature. Double immunofluorescence microscopic analyses demonstrated the coexpression of adipogenic and osteoblastic specific markers in individual cells, providing evidence for a transdifferentiation event. At the molecular level, this conversion was associated with upregulated expression levels of reprogramming genes and a decrease in the DNA methylation level. In line with these in vitro results, preliminary immunohistochemical analysis of bone sections revealed adipogenic marker expression in osteoblasts from elderly subjects. Altogether, these data suggest that osteoblast transdifferentiation could contribute to decreased bone mass upon ageing.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0245014