Isolating, identifying and evaluating of oil degradation strains for the air-assisted microbial enhanced oil recovery process

Due to the inefficient reproduction of microorganisms in oxygen-deprived environments of the reservoir, the applications of microbial enhanced oil recovery (MEOR) are restricted. To overcome this problem, a new type of air-assisted MEOR process was investigated. Three compounding oil degradation str...

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Bibliographic Details
Published in:PloS one 2021-01, Vol.16 (1), p.e0243976
Main Authors: Cheng, Mingming, Yu, Long, Gao, Jianbo, Lei, Guanglun, Zhang, Zaiwang
Format: Article
Language:English
Subjects:
Air injection
Alkanes - metabolism
Anaerobic bacteria
Bacillus - growth & development
Bacillus - isolation & purification
Bacillus - metabolism
Bacteria - growth & development
Bacteria - isolation & purification
Bacteria - metabolism
Biodegradation
Biodegradation, Environmental
Biology and Life Sciences
Carbon
Carbon sources
Decomposition (Chemistry)
Dissolved oxygen
Earth Sciences
Ecology and Environmental Sciences
Engineering
Engineering and Technology
Enhanced oil recovery
Enterobacter - growth & development
Enterobacter - isolation & purification
Enterobacter - metabolism
Environmental engineering
Experiments
Fermentation
Flood predictions
Flooding
Mathematical models
Metabolism
Metabolites
Methods
Microbial enhanced oil recovery
Microorganisms
Oil
Oil and Gas Fields - microbiology
Oil recovery
Oxygen
Oxygen - metabolism
Oxygen consumption
Permeability
Petroleum
Petroleum - microbiology
Petroleum engineering
Petroleum research
Physical Sciences
Physiological aspects
Pseudomonas - growth & development
Pseudomonas - isolation & purification
Pseudomonas - metabolism
Recovery (Medical)
Safety
Safety engineering
Salinity
Strains (organisms)
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Summary:Due to the inefficient reproduction of microorganisms in oxygen-deprived environments of the reservoir, the applications of microbial enhanced oil recovery (MEOR) are restricted. To overcome this problem, a new type of air-assisted MEOR process was investigated. Three compounding oil degradation strains were screened using biochemical experiments. Their performances in bacterial suspensions with different amounts of dissolved oxygen were evaluated. Water flooding, microbial flooding and air-assisted microbial flooding core flow experiments were carried out. Carbon distribution curve of biodegraded oil with different oxygen concentration was determined by chromatographic analysis. The long-chain alkanes are degraded by microorganisms. A simulation model was established to take into account the change in oxygen concentration in the reservoir. The results showed that the optimal dissolved oxygen concentration for microbial growth was 4.5~5.5mg/L. The main oxygen consumption in the reservoir happened in the stationary and declining phases of the microbial growth systems. In order to reduce the oxygen concentration to a safe level, the minimum radius of oxygen consumption was found to be about 145m. These results demonstrate that the air-assisted MEOR process can overcome the shortcomings of traditional microbial flooding techniques. The findings of this study can help for better understanding of microbial enhanced oil recovery and improving the efficiency of microbial oil displacement.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0243976