Activated whole-body arginine pathway in high-active mice

Our previous studies suggest that physical activity (PA) levels are potentially regulated by endogenous metabolic mechanisms such as the vasodilatory roles of nitric oxide (NO) production via the precursor arginine (ARG) and ARG-related pathways. We assessed ARG metabolism and its precursors [citrul...

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Bibliographic Details
Published in:PloS one 2020-06, Vol.15 (6), p.e0235095-e0235095
Main Authors: Granados, Jorge Z, Ten Have, Gabriella A. M, Letsinger, Ayland C, Thaden, John J, Engelen, Marielle P. K. J, Lightfoot, J. Timothy, Deutz, Nicolaas E. P, Blachier, François
Format: Article
Language:English
Subjects:
Aging
Amino acid metabolism
Arginine
Biology
Biology and Life Sciences
Body composition
Catheterization
Chromatography
Citrulline
Exercise
Gene expression
Glutamine
Kinesiology
Laboratories
Liquid chromatography
Magnetic resonance imaging
Mass spectrometry
Mass spectroscopy
Medicine and Health Sciences
Metabolism
Metabolites
Nitric oxide
Ornithine
Phenylalanine
Physical activity
Physical fitness
Physical Sciences
Physiological aspects
Physiological research
Precursors
Proteins
Stable isotopes
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Summary:Our previous studies suggest that physical activity (PA) levels are potentially regulated by endogenous metabolic mechanisms such as the vasodilatory roles of nitric oxide (NO) production via the precursor arginine (ARG) and ARG-related pathways. We assessed ARG metabolism and its precursors [citrulline (CIT), glutamine (GLN), glutamate (GLU), ornithine (ORN), and phenylalanine (PHE)] by measuring plasma concentration, whole-body production (WBP), de novo ARG and NO production, and clearance rates in previously classified low-active (LA) or high-active (HA) mice. We assessed LA (n = 23) and HA (n = 20) male mice by administering a stable isotope tracer pulse via jugular catheterization. We measured plasma enrichments via liquid chromatography tandem mass spectrometry (LC-MS/MS) and body compostion by echo-MRI. WBP, clearance rates, and de novo ARG and NO were calculated. Compared to LA mice, HA mice had lower plasma concentrations of GLU (71.1%; 36.8 ± 2.9 vs. 17.5 ± 1.7[mu]M; p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0235095