The G123 rice mutant, carrying a mutation in SE13, presents alterations in the expression patterns of photosynthetic and major flowering regulatory genes

Day length is a determinant of flowering time in rice. Phytochromes participate in flowering regulation by measuring the number of daylight hours to which the plant is exposed. Here we describe G123, a rice mutant generated by irradiation, which displays insensitivity to the photoperiod and early fl...

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Bibliographic Details
Published in:PloS one 2020-05, Vol.15 (5), p.e0233120-e0233120
Main Authors: Reig-Valiente, Juan Luis, Borredá, Carles, Talón, Manuel, Domingo, Concha
Format: Article
Language:English
Subjects:
Adaptation
Analysis
Biology and Life Sciences
Biosynthesis
Chromophores
Circadian rhythm
Day length
Daylight
EDTA
Enzymes
Flowering
Flowers & plants
Gene deletion
Gene expression
Gene mutation
Gene regulation
Genes
Genetic aspects
Genomes
Genomics
Growth
Irradiation
Light effects
Mutants
Mutation
Phenotypes
Photoperiodism
Photosynthesis
Physical Sciences
Phytochrome
Phytochromes
Plant biochemistry
Plant biology
Plant flowering
Proteins
Radiation
Research and Analysis Methods
Ribonucleic acid
Rice
RNA
Seeds
Time
Transcriptomics
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Summary:Day length is a determinant of flowering time in rice. Phytochromes participate in flowering regulation by measuring the number of daylight hours to which the plant is exposed. Here we describe G123, a rice mutant generated by irradiation, which displays insensitivity to the photoperiod and early flowering under both long day and short day conditions. To detect the mutation responsible for the early flowering phenotype exhibited by G123, we generated an F2 population, derived from crossing with the wild-type, and used a pipeline to detect genomic structural variation, initially developed for human genomes. We detected a deletion in the G123 genome that affects the PHOTOPERIOD SENSITIVITY13 (SE13) gene, which encodes a phytochromobilin synthase, an enzyme implicated in phytochrome chromophore biosynthesis. The transcriptomic analysis, performed by RNA-seq, in the G123 plants indicated an alteration in photosynthesis and other processes related to response to light. The expression patterns of the main flowering regulatory genes, such as Ghd7, Ghd8 and PRR37, were altered in the plants grown under both long day and short day conditions. These findings indicate that phytochromes are also involved in the regulation of these genes under short day conditions, and extend the role of phytochromes in flowering regulation in rice.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0233120