Acetylcholinesterase electrochemical biosensors with graphene-transition metal carbides nanocomposites modified for detection of organophosphate pesticides

An acetylcholinesterase biosensor modified with graphene and transition metal carbides was prepared to detect organophosphorus pesticides. Cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy were used to characterize the electrochemical catalysis of the bio...

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Bibliographic Details
Published in:PloS one 2020-04, Vol.15 (4), p.e0231981
Main Authors: Wang, Bo, Li, Yiru, Hu, Huaying, Shu, Wenhao, Yang, Lianqiao, Zhang, Jianhua
Format: Article
Language:English
Subjects:
Acetylcholine
Acetylcholinesterase
Acetylcholinesterase - metabolism
Biocompatibility
Biology and Life Sciences
Biosensing Techniques - methods
Biosensors
Carbides
Catalysis
Chitosan
Detection equipment
Dichlorvos
Education
Electric properties
Electrical properties
Electrochemical impedance spectroscopy
Electrochemical Techniques
Electrochemistry
Electrodes
Engineering and Technology
Enzymes
Enzymes, Immobilized - chemistry
Enzymes, Immobilized - metabolism
Glassy carbon
Graphene
Graphite
Graphite - chemistry
Laboratories
Limit of Detection
Medical equipment
Metal carbides
Metals
Metals (Materials)
Methods
Morphology
Nanocomposites
Nanocomposites - chemistry
Nanomaterials
Optimization
Organophosphates
Organophosphorus Compounds - analysis
Organophosphorus pesticides
Performance degradation
Pesticides
Pesticides - analysis
Physical Sciences
Reproducibility of Results
Research and Analysis Methods
Room temperature
Sensors
Spectroscopy
Titanium - chemistry
Transition Elements - chemistry
Transition metal compounds
Transition metals
Voltammetry
Work stations
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Summary:An acetylcholinesterase biosensor modified with graphene and transition metal carbides was prepared to detect organophosphorus pesticides. Cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy were used to characterize the electrochemical catalysis of the biosensor: acetylcholinesterase/chitosan-transition metal carbides/graphene/glassy carbon electrode. With the joint modification of graphene and transition metal carbides, the biosensor has a good performance in detecting dichlorvos with a linear relationship from 11.31 μM to 22.6 nM and the limit of detection was 14.45 nM. Under the premise of parameter optimization, the biosensor showed a good catalytic performance for acetylcholine. Compared to the biosensors without modification, it expressed a better catalytic performance due to the excellent electrical properties, biocompatibility and high specific surface area of graphene, transition metal carbides. Finally, the biosensor exhibits good stability, which can be stored at room temperature for one month without significant performance degradation, and has practical potential for sample testing.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0231981