Apocynin and catalase prevent hypertension and kidney injury in Cyclosporine A-induced nephrotoxicity in rats

Oxidative stress is involved in the pathogenesis of a number of diseases including hypertension and renal failure. There is enhanced expression of nicotinamide adenine dinucleotide (NADPH oxidase) and therefore production of hydrogen peroxide (H2O2) during renal disease progression. This study inves...

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Published in:	PloS one 2020-04, Vol.15 (4), p.e0231472
Main Authors:	Tan, Yong Chia, Abdul Sattar, Munavvar, Ahmeda, Ahmad F, Abdul Karim Khan, Nurzalina, Murugaiyah, Vikneswaran, Ahmad, Ashfaq, Hassan, Zurina, Kaur, Gurjeet, Abdulla, Mohammed Hadi, Johns, Edward James
Format:	Article
Language:	eng
Subjects:	Adenine Animal models Animal tissues Antioxidants Biology and Life Sciences Blood pressure Catalase Combined treatment Creatinine Cyclosporins Enzymes Health services Hemodynamics Hydrogen Hydrogen peroxide Hydrogen production Hypertension Inflammation Injury prevention Kidney diseases Kidneys Kinases Malondialdehyde Medical research Medicine and Health Sciences Medicine, Experimental Metabolism mRNA NAD(P)H oxidase NADPH Nicotinamide Nicotinamide adenine dinucleotide NOX4 protein Oxidase Oxidases Oxidative stress Parameters Pathogenesis Pharmaceutical sciences Physiology Renal failure RNA Scavenging Superoxide Superoxide dismutase Transplants & implants Tumor necrosis factor-TNF Urea Urine
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Summary:	Oxidative stress is involved in the pathogenesis of a number of diseases including hypertension and renal failure. There is enhanced expression of nicotinamide adenine dinucleotide (NADPH oxidase) and therefore production of hydrogen peroxide (H2O2) during renal disease progression. This study investigated the effect of apocynin, an NADPH oxidase inhibitor and catalase, an H2O2 scavenger on Cyclosporine A (CsA) nephrotoxicity in Wistar-Kyoto rats. Rats received CsA (25mg/kg/day via gavage) and were assigned to vehicle, apocynin (2.5mmol/L p.o.), catalase (10,000U/kg/day i.p.) or apocynin plus catalase for 14 days. Renal functional and hemodynamic parameters were measured every week, and kidneys were harvested at the end of the study for histological and NADPH oxidase 4 (NOX4) assessment. Oxidative stress markers and blood urea nitrogen (BUN) were measured. CsA rats had higher plasma malondialdehyde (by 340%) and BUN (by 125%), but lower superoxide dismutase and total antioxidant capacity (by 40%, all P
ISSN:	1932-6203 1932-6203