CRISPR/Cas9 gene editing in the West Nile Virus vector, Culex quinquefasciatus Say

Culex quinquefasciatus Say is an opportunistic blood feeder with a wide geographic distribution which is also a major vector for a range of diseases of both animals and humans. CRISPR/Cas technologies have been applied to a wide variety of organisms for both applied and basic research purposes. CRIS...

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Bibliographic Details
Published in:PloS one 2019-11, Vol.14 (11), p.e0224857
Main Authors: Anderson, Michelle E, Mavica, Jessica, Shackleford, Lewis, Flis, Ilona, Fochler, Sophia, Basu, Sanjay, Alphey, Luke
Format: Article
Language:English
Subjects:
Aedes - genetics
Aedes - virology
Amino Acid Sequence
Animal diseases
Animals
Arthropods
Biology and Life Sciences
CRISPR
CRISPR-Associated Protein 9 - metabolism
CRISPR-Cas Systems - genetics
Culex - genetics
Culex - virology
Culex quinquefasciatus
Deoxyribonucleic acid
DNA
Embryo, Nonmammalian - metabolism
Encephalitis
Gene Editing
Genes
Genetic modification
Genetic vectors
Genetics
Genome editing
Genomes
Geographical distribution
INDEL Mutation - genetics
KMO gene
Medicine and Health Sciences
Microinjection
Microinjections
Mosquito Vectors - genetics
Mosquito Vectors - virology
Mosquitoes
Mutagenesis
Mutation
Phenotypes
Proteins
Public health
Public health movements
Research and Analysis Methods
Technology
Tropical diseases
Viruses
West Nile fever
West Nile virus
West Nile virus - physiology
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Summary:Culex quinquefasciatus Say is an opportunistic blood feeder with a wide geographic distribution which is also a major vector for a range of diseases of both animals and humans. CRISPR/Cas technologies have been applied to a wide variety of organisms for both applied and basic research purposes. CRISPR/Cas methods open new possibilities for genetic research in non-model organisms of public health importance. In this work we have adapted microinjection techniques commonly used in other mosquito species to Culex quinquefasciatus, and have shown these to be effective at generating homozygous knock-out mutations of a target gene in one generation. This is the first description of the kmo gene and mutant phenotype in this species.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0224857