Distribution of the Duffy genotypes in Malaysian Borneo and its relation to Plasmodium knowlesi malaria susceptibility

The Duffy blood group plays a key role in Plasmodium knowlesi and Plasmodium vivax invasion into human erythrocytes. The geographical distribution of the Duffy alleles differs between regions with the FY*A allele having high frequencies in many Asian populations, the FY*B allele is found predominate...

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Bibliographic Details
Published in:PloS one 2019-09, Vol.14 (9), p.e0222681-e0222681
Main Authors: de Silva, Jeremy Ryan, Amir, Amirah, Lau, Yee Ling, Ooi, Choo-Huck, Fong, Mun Yik
Format: Article
Language:English
Subjects:
Alleles
Antigens
Binding sites
Biology and Life Sciences
Blood
Blood & organ donations
Blood Group Antigens - genetics
Blood groups
Borneo
Chemokines
Consent
Duffy Blood-Group System - genetics
Earth Sciences
Ecology and Environmental Sciences
Erythrocytes
Erythrocytes - parasitology
Gene frequency
Gene Frequency - genetics
Genetic aspects
Genetic Predisposition to Disease - genetics
Genotype
Genotypes
Geographical distribution
Health care
Homogeneity
Humans
Infections
Malaria
Malaria - blood
Malaria - genetics
Malaria - parasitology
Malaysia
Medicine
Medicine and Health Sciences
Parasitology
Patients
People and Places
Plasmodium knowlesi
Plasmodium knowlesi - pathogenicity
Plasmodium vivax - pathogenicity
Polymerase chain reaction
Population
Population genetics
Populations
Red blood cells
Research and Analysis Methods
Risk factors
Vector-borne diseases
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Summary:The Duffy blood group plays a key role in Plasmodium knowlesi and Plasmodium vivax invasion into human erythrocytes. The geographical distribution of the Duffy alleles differs between regions with the FY*A allele having high frequencies in many Asian populations, the FY*B allele is found predominately in European populations and the FY*Bes allele found predominantly in African regions. A previous study in Peninsular Malaysia indicated high homogeneity of the dominant FY*A/FY*A genotype. However, the distribution of the Duffy genotypes in Malaysian Borneo is currently unknown. In the present study, the distribution of Duffy blood group genotypes and allelic frequencies among P. knowlesi infected patients as well as healthy individuals in Malaysian Borneo were determined. A total of 79 P. knowlesi patient blood samples and 76 healthy donor samples were genotyped using allele specific polymerase chain reaction (ASP-PCR). Subsequently a P. knowlesi invasion assay was carried out on FY*AB/ FY*A and FY*A/ FY*A Duffy genotype blood to investigate if either genotype conferred increased susceptibility to P. knowlesi invasion. Our results show almost equal distribution between the homozygous FY*A/FY*A and heterozygous FY*A/FY*B genotypes. This is in stark contrast to the Duffy distribution in Peninsular Malaysia and the surrounding Southeast Asian region which is dominantly FY*A/FY*A. The mean percent invasion of FY*A/FY*A and FY*A/FY*B blood was not significantly different indicating that neither blood group confers increased susceptibility to P. knowlesi invasion.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0222681