The zebrafish HGF receptor met controls migration of myogenic progenitor cells in appendicular development

The hepatocyte growth factor receptor C-met plays an important role in cellular migration, which is crucial for many developmental processes as well as for cancer cell metastasis. C-met has been linked to the development of mammalian appendicular muscle, which are derived from migrating muscle proge...

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Bibliographic Details
Published in:PloS one 2019-07, Vol.14 (7), p.e0219259-e0219259
Main Authors: Nord, Hanna, Dennhag, Nils, Tydinger, Hanna, von Hofsten, Jonas
Format: Article
Language:English
Subjects:
Animals
Appendages
Biology
Biology and Life Sciences
c-Met protein
Cancer metastasis
Cell migration
Cell Movement - physiology
Cells (biology)
CRISPR
CRISPR-Cas Systems
Danio rerio
Extremities - growth & development
Fins
Fins (Anatomy)
Gene Expression Regulation, Developmental - genetics
Gene mutation
Genes
Genetic aspects
Genetic research
Growth factor receptors
Growth factors
Hepatocyte growth factor
Homology
Mammals
Medicine and Health Sciences
MET protein
Metastases
Muscle cells
Muscle Development - physiology
Muscle Fibers, Skeletal - metabolism
Muscle, Skeletal - metabolism
Muscles
Muscles - metabolism
Mutants
Mutation
Myogenesis
Physiological aspects
Progenitor cells
Proto-Oncogene Proteins c-met - genetics
Proto-Oncogene Proteins c-met - metabolism
Research and Analysis Methods
Stem cells
Stem Cells - metabolism
Transcription factors
Vertebrates
Zebrafish
Zebrafish - embryology
Zebrafish - genetics
Zebrafish Proteins - metabolism
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Summary:The hepatocyte growth factor receptor C-met plays an important role in cellular migration, which is crucial for many developmental processes as well as for cancer cell metastasis. C-met has been linked to the development of mammalian appendicular muscle, which are derived from migrating muscle progenitor cells (MMPs) from within the somite. Mammalian limbs are homologous to the teleost pectoral and pelvic fins. In this study we used Crispr/Cas9 to mutate the zebrafish met gene and found that the MMP derived musculature of the paired appendages was severely affected. The mutation resulted in a reduced muscle fibre number, in particular in the pectoral abductor, and in a disturbed pectoral fin function. Other MMP derived muscles, such as the sternohyoid muscle and posterior hypaxial muscle were also affected in met mutants. This indicates that the role of met in MMP function and appendicular myogenesis is conserved within vertebrates.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0219259