Characterization of genes and alleles involved in the control of flowering time in grapevine

Grapevine (Vitis vinifera) is one of the most important perennial crop plants in worldwide. Understanding of developmental processes like flowering, which impact quality and quantity of yield in this species is therefore of high interest. This gets even more important when considering some of the ex...

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Bibliographic Details
Published in:PloS one 2019-07, Vol.14 (7), p.e0214703
Main Authors: Kamal, Nadia, Ochßner, Iris, Schwandner, Anna, Viehöver, Prisca, Hausmann, Ludger, Töpfer, Reinhard, Weisshaar, Bernd, Holtgräwe, Daniela
Format: Article
Language:English
Subjects:
Alleles
Arabidopsis thaliana
Binding sites
Bioinformatics
Biology
Biology and Life Sciences
Biotechnology
Breeding
Chromosome 1
Chromosome Mapping
Chromosomes
Climate change
Climatic conditions
Computational biology
DNA sequencing
Flowering
Flowers & plants
Flowers - genetics
Flowers - growth & development
Fruits
Gene expression
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Gene mapping
Gene sequencing
Genes
Genes, Plant
Genetic aspects
Genomes
Genomics
Genotypes
Global temperature changes
Grapes
Grapevines
Haplotypes
Homology
Kinases
Mapping
Methods
Phenotypes
Physiological aspects
Plant Breeding
Plant flowering
Plants (botany)
Population genetics
Population studies
Quantitative genetics
Quantitative Trait Loci
Research and Analysis Methods
Ripening
Vitis - genetics
Vitis - growth & development
Vitis vinifera
Wine
Wines
Workflow
Workflow software
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Summary:Grapevine (Vitis vinifera) is one of the most important perennial crop plants in worldwide. Understanding of developmental processes like flowering, which impact quality and quantity of yield in this species is therefore of high interest. This gets even more important when considering some of the expected consequences of climate change. Earlier bud burst and flowering, for example, may result in yield loss due to spring frost. Berry ripening under higher temperatures will impact wine quality. Knowledge of interactions between a genotype or allele combination and the environment can be used for the breeding of genotypes that are better adapted to new climatic conditions. To this end, we have generated a list of more than 500 candidate genes that may play a role in the timing of flowering. The grapevine genome was exploited for flowering time control gene homologs on the basis of functional data from model organisms like A. thaliana. In a previous study, a mapping population derived from early flowering GF.GA-47-42 and late flowering 'Villard Blanc' was analyzed for flowering time QTLs. In a second step we have now established a workflow combining amplicon sequencing and bioinformatics to follow alleles of selected candidate genes in the F1 individuals and the parental genotypes. Allele combinations of these genes in individuals of the mapping population were correlated with early or late flowering phenotypes. Specific allele combinations of flowering time candidate genes within and outside of the QTL regions for flowering time on chromosome 1, 4, 14, 17, and 18 were found to be associated with an early flowering phenotype. In addition, expression of many of the flowering candidate genes was analyzed over consecutive stages of bud and inflorescence development indicating functional roles of these genes in the flowering control network.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0214703