Catechin attenuates TNF-α induced inflammatory response via AMPK-SIRT1 pathway in 3T3-L1 adipocytes

Chronic inflammation is a fundamental symptom of many diseases. Catechin possesses anti-oxidant and anti-inflammatory properties. However, the mechanism of catechin to prevent inflammation in 3T3-L1 adipocytes caused by TNF-α remains unknown. Therefore, the effects of catechin on the gene expression...

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Bibliographic Details
Published in:PloS one 2019-05, Vol.14 (5), p.e0217090-e0217090
Main Authors: Cheng, An-Wei, Tan, Xin, Sun, Jin-Yue, Gu, Chun-Mei, Liu, Chao, Guo, Xu
Format: Article
Language:English
Subjects:
3T3-L1 Cells
Adipocytes
Adipocytes - drug effects
Adipocytes - immunology
Adipocytes - metabolism
Adipogenesis
Agriculture
AMP-Activated Protein Kinases - genetics
AMP-Activated Protein Kinases - metabolism
Animals
Anti-inflammatory agents
Biochemistry
Biology and Life Sciences
Cascades
Catechin
Catechin - pharmacology
Cell activation
Cell viability
Cholecystokinin
Cyclooxygenase-2
Cytokines
Cytokines - metabolism
Energy
Enzymes
Flavonoids
Food science
FOXO3 protein
Gene expression
Gene Expression Regulation - drug effects
IL-1β
Inflammation
Inflammation - chemically induced
Inflammation - metabolism
Inflammation - pathology
Inflammation - prevention & control
Inflammatory response
Inhibition
Insulin resistance
Interleukin 10
Interleukin 12
Interleukin 4
Interleukin 6
Kinases
Laboratories
Medicine and Health Sciences
Metabolism
Mice
NF-kappa B - genetics
NF-kappa B - metabolism
NF-κB protein
Nitric-oxide synthase
Obesity
Phosphorylation
Polymerase chain reaction
Polyphenols
Proteins
Signal Transduction
SIRT1 protein
Sirtuin 1 - genetics
Sirtuin 1 - metabolism
Tumor Necrosis Factor-alpha - toxicity
Tumor necrosis factor-TNF
Tumor necrosis factor-α
Western blotting
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Summary:Chronic inflammation is a fundamental symptom of many diseases. Catechin possesses anti-oxidant and anti-inflammatory properties. However, the mechanism of catechin to prevent inflammation in 3T3-L1 adipocytes caused by TNF-α remains unknown. Therefore, the effects of catechin on the gene expression of cytokines and the activation of cell signals in TNF-α induced 3T3-L1 adipocytes were investigated. The effects of catechin on adipogenesis and cell viability were detected by Oil Red O staining and CCK-8 assay, respectively. The genes expression of cytokines was determined by real-time RT-PCR. The expression of NF-κB, AMPK, FOXO3a and SIRT1 on translation level was determined by western blotting analysis. The results demonstrated that catechin significantly enhanced adipogenesis and cell viability. catechin inhibited the gene expression of pro-inflammatory cytokines including IL-1α, IL-1β, IL-6, IL-12p35, and inflammatory enzymes including iNOS and COX-2, but enhanced the gene expression of anti-inflammatory cytokines including IL-4 and IL-10. Catechin also inhibited the activation of NF-κB, AMPK, FOXO3a and SIRT1, but increased the phosphorylation level of the above factors. All these results indicated that as a potential therapeutic strategy catechin has the ability of attenuating inflammatory response triggered by TNF-α through signaling cascades involved in inflammation and cytokines.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0217090