The protective effect of non-invasive low intensity pulsed electric field and fucoidan in preventing oxidative stress-induced motor neuron death via ROCK/Akt pathway

With the expansion of the aged population, it is predicted that neurodegenerative diseases (NDDs) will become a major threat to public health worldwide. However, existing therapies can control the symptoms of the diseases at best, rather than offering a fundamental cure. As for the complex pathogene...

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Bibliographic Details
Published in:PloS one 2019-03, Vol.14 (3), p.e0214100-e0214100
Main Authors: Hsieh, Chih-Hsiung, Lu, Chueh-Hsuan, Kuo, Yu-Yi, Lin, Guan-Bo, Chao, Chih-Yu
Format: Article
Language:English
Subjects:
AKT protein
Amyotrophic lateral sclerosis
Animals
Biology and Life Sciences
Biomedical engineering
Care and treatment
Cell adhesion & migration
Cell cycle
Cell death
Cell Death - drug effects
Cell Line
Degeneration
Disease
Drug metabolism
EDTA
Electric fields
Electric Stimulation Therapy
Engineering
Free radicals
Fucoidan
Health aspects
Health physics
Health risks
Humans
Hydrogen peroxide
Kinases
Medical research
Medicine
Medicine and Health Sciences
Metabolism
Mice
Motor Neurons - metabolism
Motor Neurons - pathology
Motors
Nervous system
Nervous system diseases
Neuroblastoma
Neurodegeneration
Neurodegenerative diseases
Neurodegenerative Diseases - metabolism
Neurodegenerative Diseases - pathology
Neurodegenerative Diseases - therapy
Neurological diseases
Neurons
Neurophysiology
Novels
Oxidative stress
Oxidative Stress - drug effects
Pathogenesis
Phosphorylation
Physics
Polysaccharides - pharmacology
Proteins
Proto-Oncogene Proteins c-akt - metabolism
Public health
Research and Analysis Methods
rho-Associated Kinases - metabolism
Risk factors
ROCK protein
Rocks
Signal Transduction - drug effects
Signs and symptoms
Spinal cord
Therapeutic applications
Ultrasound
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Summary:With the expansion of the aged population, it is predicted that neurodegenerative diseases (NDDs) will become a major threat to public health worldwide. However, existing therapies can control the symptoms of the diseases at best, rather than offering a fundamental cure. As for the complex pathogenesis, clinical and preclinical researches have indicated that oxidative stress, a central role in neuronal degeneration, is a possible therapeutic target in the development of novel remedies. In this study, the motor neuron-like cell line NSC-34 was employed as an experimental model in probing the effects induced by the combination of non-invasive low intensity pulsed electric field (LIPEF) and fucoidan on the H2O2-induced neuron damage. It was found that single treatment of the LIPEF could protect the NSC-34 cells from oxidative stress, and the protective effect was enhanced by combining the LIPEF and fucoidan. Notably, it was observed that single treatment of the LIPEF obviously suppressed the H2O2-enhanced expression of ROCK protein and increased the phosphorylation of Akt in the H2O2-treated NSC-34 cells. Moreover, the LIPEF can be easily modified to concentrate on a specific area. Accordingly, this technique can be used as an advanced remedy for ROCK inhibition without the drawback of drug metabolism. Therefore, we suggest the LIPEF would be a promising strategy as a treatment for motor neurodegeneration and warrant further probe into its potential in treating other neuronal degenerations.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0214100