Growth substrate may influence biofilm susceptibility to antibiotics

The CDC biofilm reactor is a robust culture system with high reproducibility in which biofilms can be grown for a wide variety of analyses. Multiple material types are available as growth substrates, yet data from biofilms grown on biologically relevant materials is scarce, particularly for antibiot...

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Bibliographic Details
Published in:PloS one 2019-03, Vol.14 (3), p.e0206774-e0206774
Main Authors: Williams, Dustin L, Smith, Scott R, Peterson, Brittany R, Allyn, Gina, Cadenas, Lousili, Epperson, Richard Tyler, Looper, Ryan E
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotics
Antimicrobial agents
Bacteria - drug effects
Bacteria - growth & development
Biofilms
Biofilms - drug effects
Biofilms - growth & development
Biology and Life Sciences
Bioreactors - microbiology
Collagen
Collagen - chemistry
Collagen - metabolism
Comparative analysis
Dosage and administration
Drug resistance
Extracellular matrix
Gram-positive bacteria
Growth
In vivo methods and tests
Materials information
Medicine and Health Sciences
Methicillin
Methods
Microbial mats
Physical Sciences
Polycarbonate
Polycarbonates
Polycarboxylate Cement - chemistry
Polycarboxylate Cement - metabolism
Reactors
Reproducibility
Salt
Streptococcus infections
Substrates
Veterans
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Summary:The CDC biofilm reactor is a robust culture system with high reproducibility in which biofilms can be grown for a wide variety of analyses. Multiple material types are available as growth substrates, yet data from biofilms grown on biologically relevant materials is scarce, particularly for antibiotic efficacy against differentially supported biofilms. In this study, CDC reactor holders were modified to allow growth of biofilms on collagen, a biologically relevant substrate. Susceptibility to multiple antibiotics was compared between biofilms of varying species grown on collagen versus standard polycarbonate coupons. Data indicated that in 13/18 instances, biofilms on polycarbonate were more susceptible to antibiotics than those on collagen, suggesting that when grown on a complex substrate, biofilms may be more tolerant to antibiotics. These outcomes may influence the translatability of antibiotic susceptibility profiles that have been collected for biofilms on hard plastic materials. Data may also help to advance information on antibiotic susceptibility testing of biofilms grown on biologically relevant materials for future in vitro and in vivo applications.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0206774