Up-regulation of antioxidative proteins TRX1, TXNL1 and TXNRD1 in the cortex of PTZ kindling seizure model mice

Oxidative stress has been considered as one of pathogenesis of brain damage led by epilepsy. Reducing oxidative stress can ameliorate brain damage during seizures. However, expression levels of important antioxidative enzymes such as thioredoxin-1 (TRX1), thioredoxin-like 1 protein (TXNL1) and thior...

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Bibliographic Details
Published in:PloS one 2019-01, Vol.14 (1), p.e0210670-e0210670
Main Authors: Yu, Jia-Tian, Liu, Ye, Dong, Ping, Cheng, Run-En, Ke, Shao-Xi, Chen, Kai-Qin, Wang, Jing-Jing, Shen, Zhong-Shan, Tang, Qiong-Yao, Zhang, Zhe
Format: Article
Language:English
Subjects:
Anesthesiology
Animals
Biology and Life Sciences
Brain
Brain damage
Brain injury
Care and treatment
Cerebellum
Convulsions & seizures
Diencephalon
Disease Models, Animal
Enzymes
Epilepsy
Gene expression
Gene mutation
International markets
Kindling
Kindling, Neurologic - genetics
Kindling, Neurologic - metabolism
Laboratories
Male
Medicine and Health Sciences
Mice
Nervous system
Oxidative stress
Pathogenesis
Phosphatase
Proteins
Reductase
Research and Analysis Methods
Risk factors
Rodents
Seizing
Seizures
Seizures - genetics
Seizures - metabolism
Thioredoxin
Thioredoxin Reductase 1 - genetics
Thioredoxin Reductase 1 - metabolism
Thioredoxins - genetics
Thioredoxins - metabolism
Up-regulation
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Summary:Oxidative stress has been considered as one of pathogenesis of brain damage led by epilepsy. Reducing oxidative stress can ameliorate brain damage during seizures. However, expression levels of important antioxidative enzymes such as thioredoxin-1 (TRX1), thioredoxin-like 1 protein (TXNL1) and thioredoxin reductase 1 (TXNRD1) during seizures have not been investigated. In this study, we examined protein and mRNA expression levels of TRX1, TXNL1 and TXNRD1 in different brain regions in PTZ induced seizure model mice. We found that protein expression levels of TRX1, TXNL1 and TXNRD1 are simultaneously up-regulated by 2- or 3-fold in the cortex of both acute and chronic seizure model mice. But there is no unified expression pattern change of these enzymes in the hippocampus, cerebellum and diencephalon in the seizure model mice. Less extent up-regulation of mRNA expression of these enzymes were also observed in the cortex of seizure mice. These data suggest that antioxidative enzymes may provide a protective effect against oxidative stress in the cortex during seizures.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0210670