Human umbilical cord blood plasma as an alternative to animal sera for mesenchymal stromal cells in vitro expansion - A multicomponent metabolomic analysis

Mesenchymal Stromal cells (MSCs) have a potential role in cell-based therapies. Foetal bovine serum (FBS) is used to supplement the basal cell culture medium but presents several disadvantages and risks. Other alternatives have been studied, including human umbilical cord blood plasma (hUCBP), aimin...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2018-10, Vol.13 (10), p.e0203936-e0203936
Main Authors: Caseiro, A R, Ivanova, G, Pedrosa, S S, Branquinho, M V, Georgieva, P, Barbosa, P P, Santos, J D, Magalhães, R, Teixeira, P, Pereira, T, Maurício, A C
Format: Article
Language:English
Subjects:
Alanine
Amino acids
Apoptosis
Biology and Life Sciences
Blood
Blood plasma
Bone marrow
Cell culture
Chain branching
Cord blood
Dental pulp
Dietary supplements
Gene expression
Glucose
Health aspects
Lactic acid
Lipid metabolism
Lipids
Magnetic resonance
Mesenchymal stem cells
Mesenchyme
Metabolism
Metabolites
Metabolomics
Multivariate statistical analysis
NMR
Nuclear magnetic resonance
Nuclear magnetic resonance spectroscopy
Nucleotides
Performance enhancement
Physical Sciences
Physiology
Research and analysis methods
Senescence
Spectroscopy
Spectrum analysis
Statistical analysis
Stem cell transplantation
Stem cells
Stromal cells
Substitutes
Supplements
Surface markers
Umbilical cord
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mesenchymal Stromal cells (MSCs) have a potential role in cell-based therapies. Foetal bovine serum (FBS) is used to supplement the basal cell culture medium but presents several disadvantages and risks. Other alternatives have been studied, including human umbilical cord blood plasma (hUCBP), aiming at the development of xeno-free culturing protocols. A comparative characterization of multicomponent metabolic composition of hUCBP and commercial FBS based on Nuclear Magnetic Resonance (NMR) spectroscopy and multivariate statistical analysis was performed. The analysis of 1H-NMR spectra revealed both similarities and differences between the two proposed supplements. Similar metabolites (amino acids, glucose, lipids and nucleotides) were found in the hUCBP and FBS NMR spectra. The results show that the major difference between the metabolic profiles of the two proposed supplements are due to the significantly higher levels of glucose and lower levels of lactate, glutamate, alanine and branched chain amino acids in hUCBP. Similar or slightly different levels of important proteinogenic amino acids, as well as of nucleotides, lipids were found in the hUCBP and FBS. In order to validate it's suitability for cell culture, umbilical cord-MSCs (UC-MSCs) and dental pulp stem cells (DPSCs) were expanded using hUCBP. In both hMSCs, in vitro culture with hUCBP supplementation presented similar to improved metabolic performances when compared to FBS. The two cell types tested expressed different optimum hUCBP percentage content. For DPSCs, the optimum hUCBP content was 6% and for UC-MSCs, 4%. Cultured hMSCs displayed no changes in senescence indicators, as well as maintained characteristic surface marker's expression. FBS substitution was associated with an increase in early apoptosis events, in a dose dependent manner, as well as to slight up- and down-regulation of targeted gene's expression. Tri-lineage differentiation capacity was also influenced by the substitution of FBS by hUCBP.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0203936