Hepatoprotective potential of Malvaviscus arboreus against carbon tetrachloride-induced liver injury in rats

Malvaviscus arboreus Cav. is a medicinal plant belonging to family Malvaceae with both ethnomedical and culinary value; however, its phytochemical and biological profiles have been scarcely studied. Accordingly, this work was designed to explore the chemical composition and the hepatoprotective pote...

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Bibliographic Details
Published in:PloS one 2018-08, Vol.13 (8), p.e0202362
Main Authors: Abdelhafez, Omnia Hesham, Fawzy, Michael Atef, Fahim, John Refaat, Desoukey, Samar Yehia, Krischke, Markus, Mueller, Martin J, Abdelmohsen, Usama Ramadan
Format: Article
Language:English
Subjects:
Acetic acid
Acids
Administration, Oral
Alanine
Alanine transaminase
Alcohol
Alkaline phosphatase
Analysis
Animals
Antioxidants
Aspartate transaminase
Bilirubin
Biology and Life Sciences
Carbon tetrachloride
Carbon Tetrachloride - adverse effects
Care and treatment
Chemical analysis
Chemical and Drug Induced Liver Injury - drug therapy
Chemical and Drug Induced Liver Injury - metabolism
Chemical and Drug Induced Liver Injury - pathology
Chemical composition
Chemical properties
Chemicals
Chromatography
Dichloromethane
Ethnomethodology
Ethyl acetate
Flavonoids
Food
Hepatotoxicity
Injuries
Injury prevention
Ionization
Liquid chromatography
Liver
Liver - drug effects
Liver - injuries
Liver - metabolism
Liver - pathology
Liver diseases
Male
Malondialdehyde
Malvaceae
Malvaceae - chemistry
Malvaceae - metabolism
Malvaviscus arboreus
Mass spectrometry
Mass spectroscopy
Medicinal plants
Metabolism
Metabolites
Metabolome
Metabolomics
Methylene chloride
NMR
Nuclear magnetic resonance
Oils & fats
Olive oil
Oral administration
Organic chemistry
Oxidative stress
People and Places
Petroleum ether
Pharmaceuticals
Pharmacy
Phenolic acids
Phenols
Physical Sciences
Phytochemicals
Phytotherapy
Plant Extracts - chemistry
Plant Extracts - pharmacology
Plant Leaves - chemistry
Plant Leaves - metabolism
Plant Stems - chemistry
Plant Stems - metabolism
Plants, Medicinal - chemistry
Plants, Medicinal - metabolism
Properties
Protective Agents - pharmacology
Ramadan
Random Allocation
Rats
Research and Analysis Methods
Silymarin
Solvents
Transaminase
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Summary:Malvaviscus arboreus Cav. is a medicinal plant belonging to family Malvaceae with both ethnomedical and culinary value; however, its phytochemical and biological profiles have been scarcely studied. Accordingly, this work was designed to explore the chemical composition and the hepatoprotective potential of M. arboreus against carbon tetrachloride (CCl4)-induced hepatotoxicity. The total extract of the aerial parts and its derived fractions (petroleum ether, dichloromethane, ethyl acetate, and aqueous) were orally administered to rats for six consecutive days, followed by injection of CCl4 (1:1 v/v, in olive oil, 1.5 ml/kg, i.p.) on the next day. Results showed that the ethyl acetate and dichloromethane fractions significantly alleviated liver injury in rats as indicated by the reduced levels of alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total bilirubin (TB), and malondialdehyde (MDA), along with enhancement of the total antioxidant capacities of their livers, with the maximum effects were recorded by the ethyl acetate fraction. Moreover, the protective actions of both fractions were comparable to those of silymarin (100 mg/kg), and have been also substantiated by histopathological evaluations. On the other hand, liquid chromatography-high resolution electrospray ionization mass spectrometry (LC‒HR‒ESI‒MS) metabolomic profiling of the crude extract of M. arboreus aerial parts showed the presence of a variety of phytochemicals, mostly phenolics, whereas the detailed chemical analysis of the most active fraction (i.e. ethyl acetate) resulted in the isolation and identification of six compounds for the first time in the genus, comprising four phenolic acids; β-resorcylic, caffeic, protocatechuic, and 4-hydroxyphenylacetic acids, in addition to two flavonoids; trifolin and astragalin. Such phenolic principles, together with their probable synergistic antioxidant and liver-protecting properties, seem to contribute to the observed hepatoprotective potential of M. arboreus.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0202362