Antimicrobial Susceptibility of Bordetella bronchiseptica Isolates from Swine and Companion Animals and Detection of Resistance Genes

Bordetella bronchiseptica causes infections of the respiratory tract in swine and other mammals and is a precursor for secondary infections with Pasteurella multocida. Treatment of B. bronchiseptica infections is conducted primarily with antimicrobial agents. Therefore it is essential to get an over...

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Bibliographic Details
Published in:PloS one 2015-08, Vol.10 (8), p.e0135703-e0135703
Main Authors: Prüller, Sandra, Rensch, Ulrike, Meemken, Diana, Kaspar, Heike, Kopp, Peter A, Klein, Günter, Kehrenberg, Corinna
Format: Article
Language:English
Subjects:
Amides
Ampicillin
Animals
Anti-Bacterial Agents - pharmacology
Antibiotics
Antiinfectives and antibacterials
Antimicrobial agents
Bacteria
beta-Lactams - pharmacology
Bordetella bronchiseptica
Bordetella bronchiseptica - drug effects
Cefotaxime
Cephalosporins - pharmacology
Dilution
Disease
Disease susceptibility
Epidemiology
Farms
Florfenicol
Food quality
Food safety
Genes
Genetic research
Health aspects
Horses
Infection
Infections
Laboratories
Lactams
Livestock
Microbial Sensitivity Tests
Pasteurella multocida
Pets - microbiology
Plasmids
Respiratory tract
Respiratory tract diseases
Swine
Swine - microbiology
Thiamphenicol - analogs & derivatives
Thiamphenicol - pharmacology
Veterinary medicine
β-Lactam antibiotics
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Summary:Bordetella bronchiseptica causes infections of the respiratory tract in swine and other mammals and is a precursor for secondary infections with Pasteurella multocida. Treatment of B. bronchiseptica infections is conducted primarily with antimicrobial agents. Therefore it is essential to get an overview of the susceptibility status of these bacteria. The aim of this study was to comparatively analyse broth microdilution susceptibility testing according to CLSI recommendations with an incubation time of 16 to 20 hours and a longer incubation time of 24 hours, as recently proposed to obtain more homogenous MICs. Susceptibility testing against a panel of 22 antimicrobial agents and two fixed combinations was performed with 107 porcine isolates from different farms and regions in Germany and 43 isolates obtained from companion animals in Germany and other European countries. Isolates with increased MICs were investigated by PCR assays for the presence of resistance genes. For ampicillin, all 107 porcine isolates were classified as resistant, whereas only a single isolate was resistant to florfenicol. All isolates obtained from companion animals showed elevated MICs for β-lactam antibiotics and demonstrated an overall low susceptibility to cephalosporines. Extension of the incubation time resulted in 1-2 dilution steps higher MIC50 values of porcine isolates for seven antimicrobial agents tested, while isolates from companion animals exhibited twofold higher MIC50/90 values only for tetracycline and cefotaxime. For three antimicrobial agents, lower MIC50 and MIC90 values were detected for both, porcine and companion animal isolates. Among the 150 isolates tested, the resistance genes blaBOR-1 (n = 147), blaOXA-2, (n = 4), strA and strB (n = 17), sul1 (n = 10), sul2 (n = 73), dfrA7 (n = 3) and tet(A) (n = 8) were detected and a plasmid localisation was identified for several of the resistance genes.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0135703