Behavioral responses of Rhodnius prolixus to volatile organic compounds released in vitro by bacteria isolated from human facial skin

Previous studies have demonstrated the role of volatile organic compounds (VOCs) produced by skin microbiota in the attraction of mosquitoes to humans. Recently, behavioral experiments confirmed the importance of VOCs released by skin microbiota in the attraction of Rhodnius prolixus (Hemiptera: Tri...

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Bibliographic Details
Published in:PLoS neglected tropical diseases 2018-04, Vol.12 (4), p.e0006423-e0006423
Main Authors: Tabares, Marcela, Ortiz, Mario, Gonzalez, Mabel, Carazzone, Chiara, Vives Florez, Martha J, Molina, Jorge
Format: Article
Language:English
Subjects:
Analysis
Anopheles gambiae
Aquatic insects
Attraction
Bacteria
Behavioural responses
Biology and Life Sciences
Brevibacterium
Chagas disease
Citrobacter
Citrobacter koseri
Corynebacterium
Culicidae
Disease transmission
Gas chromatography
Headspace
Hemiptera
Human behavior
Insects
Laboratories
Mass spectrometry
Mass spectroscopy
Medicine and Health Sciences
Microbiota
Microbiota (Symbiotic organisms)
Mosquitoes
Multivariate analysis
Natural products
Odors
Organic compounds
Physical Sciences
Physiological aspects
Research and Analysis Methods
Rhodnius prolixus
Risk factors
Skin
Solid phase methods
Staphylococcus
Staphylococcus epidermidis
Supervision
Transmission
Triatominae
Tropical diseases
Vector-borne diseases
VOCs
Volatile organic compounds
Volunteers
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Description
Summary:Previous studies have demonstrated the role of volatile organic compounds (VOCs) produced by skin microbiota in the attraction of mosquitoes to humans. Recently, behavioral experiments confirmed the importance of VOCs released by skin microbiota in the attraction of Rhodnius prolixus (Hemiptera: Triatominae), a vector of Chagas disease. In this study, we screened for VOCs released in vitro by bacteria isolated from human facial skin that were able to elicit behavioral responses in R. prolixus. The VOCs released in vitro by eight bacterial species during two growth phases were tested with adult Rhodnius prolixus insects using a dual-choice "T"-shaped olfactometer. In addition, the VOCs released by the bacteria were analyzed with headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS). The VOCs produced by Staphylococcus capitis 11C, Staphylococcus warneri and Staphylococcus epidermidis 1 were attractive to R. prolixus, while the VOCs released by Citrobacter koseri 6P, Brevibacterium epidermidis and Micrococcus luteus 23 were non-attractive. The results shown here indicate that VOCs released by bacteria isolated from human facial skin have a potential for biotechnological uses as a strategy to prevent the vectorial transmission of Chagas disease mediated by Rhodnius prolixus.
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0006423