Dietary challenges differentially affect activity and sleep/wake behavior in mus musculus: Isolating independent associations with diet/energy balance and body weight

Associated with numerous metabolic and behavioral abnormalities, obesity is classified by metrics reliant on body weight (such as body mass index). However, overnutrition is the common cause of obesity, and may independently contribute to these obesity-related abnormalities. Here, we use dietary cha...

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Bibliographic Details
Published in:PloS one 2018-05, Vol.13 (5), p.e0196743
Main Authors: Perron, Isaac J, Keenan, Brendan T, Chellappa, Karthikeyani, Lahens, Nicholas F, Yohn, Nicole L, Shockley, Keith R, Pack, Allan I, Veasey, Sigrid C
Format: Article
Language:English
Subjects:
Abnormalities
Activity patterns
Agouti-Related Protein - metabolism
Analysis
Animal behavior
Animal models
Animals
Behavior, Animal - physiology
Biology and Life Sciences
Biomarkers
Biomarkers - blood
Blood
Body mass
Body mass index
Body size
Body weight
Body Weight - physiology
Circadian Rhythm - physiology
Diet
Diet - methods
Energy balance
Energy Intake - physiology
Energy Metabolism - physiology
Gene expression
Gene Expression - physiology
Gene sequencing
Health aspects
Hormones
Hypothalamus
Hypothalamus - metabolism
Hypothalamus - physiology
Insulin
Leptin
Leptin - blood
Male
Medicine and Health Sciences
Mice
Mice, Inbred C57BL
Neuropeptide Y
Neuropeptide Y - metabolism
Obesity
Obesity - blood
Obesity - metabolism
Obesity - physiopathology
Occupational health
Overnutrition
Regression analysis
Regression models
Ribonucleic acid
RNA
Rodents
Sleep
Sleep - physiology
Sleep and wakefulness
Wakefulness - physiology
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Summary:Associated with numerous metabolic and behavioral abnormalities, obesity is classified by metrics reliant on body weight (such as body mass index). However, overnutrition is the common cause of obesity, and may independently contribute to these obesity-related abnormalities. Here, we use dietary challenges to parse apart the relative influence of diet and/or energy balance from body weight on various metabolic and behavioral outcomes. Seventy male mice (mus musculus) were subjected to the diet switch feeding paradigm, generating groups with various body weights and energetic imbalances. Spontaneous activity patterns, blood metabolite levels, and unbiased gene expression of the nutrient-sensing ventral hypothalamus (using RNA-sequencing) were measured, and these metrics were compared using standardized multivariate linear regression models. Spontaneous activity patterns were negatively related to body weight (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0196743