BaiCD gene cluster abundance is negatively correlated with Clostridium difficile infection

Clostridium difficile infection (CDI) is a major cause of hospital-acquired diarrhea. Secondary bile acids were shown to confer resistance to colonization by C. difficile. 7α-dehydroxylation is a key step in transformation of primary to secondary bile acids and required genes have been located in a...

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Bibliographic Details
Published in:PloS one 2018-05, Vol.13 (5), p.e0196977
Main Authors: Solbach, Philipp, Chhatwal, Patrick, Woltemate, Sabrina, Tacconelli, Evelina, Buhl, Michael, Gerhard, Markus, Thoeringer, Christoph K, Vehreschild, Maria J G T, Jazmati, Nathalie, Rupp, Jan, Manns, Michael P, Bachmann, Oliver, Suerbaum, Sebastian
Format: Article
Language:English
Subjects:
Abundance
Acids
Anti-Bacterial Agents - therapeutic use
Antibiotics
Bacteria
Bacterial Proteins - genetics
Bacterial Toxins
Bile
Bile - microbiology
Bile acids
Bile Acids and Salts - biosynthesis
Bile Acids and Salts - genetics
Biology and Life Sciences
Biosynthesis
Clostridioides difficile - genetics
Clostridioides difficile - pathogenicity
Clostridium
Clostridium difficile
Clostridium infections
Clostridium Infections - genetics
Clostridium Infections - microbiology
Clostridium Infections - transmission
Clusters
Colonization
Diarrhea
Diarrhea - genetics
Diarrhea - microbiology
Endocrinology
Epidemiology
Fecal Microbiota Transplantation
Fecal microflora
Feces
Feces - microbiology
Female
Gastroenterology
Genes
Genetic aspects
Genetic transformation
Hepatology
Hospitals
Humans
Hygiene
Immunology
Infections
Infectious diseases
Internal medicine
Male
Medical schools
Medicine and Health Sciences
Microbiota
Microbiota - genetics
Patients
Physiology
Research and Analysis Methods
Sampling methods
Transformation
Transplantation
Transplants & implants
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Summary:Clostridium difficile infection (CDI) is a major cause of hospital-acquired diarrhea. Secondary bile acids were shown to confer resistance to colonization by C. difficile. 7α-dehydroxylation is a key step in transformation of primary to secondary bile acids and required genes have been located in a single bile acid-inducible (bai) operon in C. scindens as well as in C. hiranonis, two Clostridium sp. recently reported to protect against C. difficile colonization. To analyze baiCD gene abundance in C. difficile positive and negative fecal samples. A species-specific qPCR for detecting baiCD genes was established. Fecal samples of patients with CDI, asymptomatic toxigenic C. difficile colonization (TCD), non-toxigenic C. difficile colonization (NTCD), of C. difficile negative (NC) patients, and of two patients before and after fecal microbiota transplantation (FMT) for recurrent CDI (rCDI) were tested for the presence of the baiCD genes. The prevalence of the baiCD gene cluster was significantly higher in C. difficile negative fecal samples than in samples of patients diagnosed with CDI (72.5% (100/138) vs. 35.9% (23/64; p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0196977