Genomic evolution of Staphylococcus aureus isolates colonizing the nares and progressing to bacteremia

Nasal colonization by Staphylococcus aureus is a key risk factor for bacteremia. The objective of this study is to identify genomic modifications occurring in nasal carriage strains of S. aureus as they progress to bacteremia in a cohort of hospitalized patients. Eight patients with S. aureus bacter...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2018-05, Vol.13 (5), p.e0195860-e0195860
Main Authors: Benoit, Jeanne B, Frank, Daniel N, Bessesen, Mary T
Format: Article
Language:English
Subjects:
Agr protein
Bacteremia
Biology and Life Sciences
Colonization
Deoxyribonucleic acid
Development and progression
DNA
Genetic aspects
Genetic testing
Glycerol
Gram-positive bacteria
Health aspects
Hospital patients
Hospitals
Identification methods
Infectious diseases
Medicine and Health Sciences
Mortality
Mutation
Nosocomial infection
Pathogenesis
Pathogens
Patients
Physiological aspects
Research and Analysis Methods
Risk analysis
Risk factors
Single-nucleotide polymorphism
Staphylococcus aureus
Staphylococcus infections
Veterans
Virulence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nasal colonization by Staphylococcus aureus is a key risk factor for bacteremia. The objective of this study is to identify genomic modifications occurring in nasal carriage strains of S. aureus as they progress to bacteremia in a cohort of hospitalized patients. Eight patients with S. aureus bacteremia were identified. Genomic sequences of the bloodstream isolates were compared with 57 nasal isolates collected longitudinally prior to the occurrence of bacteremia, which covered a timespan of up to 326 days before bacteremia. Within each subject, nasal colonizing strains were closely related to bacteremia strains. Within a subject, the number of single nucleotide polymorphisms (SNPs) observed between time points was greater than within a single time point. Co-colonization and strain replacement were observed in one case. In all cases colonization progressed to bacteremia without addition of new virulence genes. In one case, a mutation in the accessory gene regulator gene caused abrogation of agr function. S. aureus evolves in the human nares at a variable rate. Progression of S. aureus nasal colonization to nosocomial infection is seldom associated with acquisition of new virulence determinants. Mutation in the agr gene with abrogation of function was associated with progression to bacteremia in one case.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0195860