Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma

Picroside II isolated from Pseudolysimachion rotundum var. subintegrum has been used as traditional medicine to treat inflammatory diseases. In this study, we assessed whether picroside II has inhibitory effects on airway inflammation in a mouse model of house dust mite (HDM)-induced asthma. In the...

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Published in:PloS one 2016-11, Vol.11 (11), p.e0167098-e0167098
Main Authors: Choi, Jin, Choi, Bo Kyong, Kim, Jin Seok, Lee, Jae-Won, Park, Hyun Ah, Ryu, Hyung Won, Lee, Su Ui, Hwang, Kwang Woo, Yun, Won-Kee, Kim, Hyoung-Chin, Ahn, Kyung-Seop, Oh, Sei-Ryang, Lee, Hyun-Jun
Format: Article
Language:English
Subjects:
Airway management
Alveoli
Analysis
Animal tissues
Animals
Asthma
Asthma - chemically induced
Asthma - immunology
Asthma - pathology
Asthma - prevention & control
Biology and Life Sciences
Biotechnology
Bronchus
Chronic obstructive pulmonary disease
Cinnamates - chemistry
Cinnamates - isolation & purification
Cinnamates - pharmacology
Cytokines
Cytokines - immunology
Dermatophagoides pteronyssinus
Disease
Disease Models, Animal
Enzyme-linked immunosorbent assay
Female
GATA-3 protein
GATA3 Transcription Factor - immunology
Gene expression
Gene Expression Regulation - drug effects
Gene Expression Regulation - immunology
Helper cells
House dust
Immunoglobulin E
Immunoglobulin G
Inflammation
Inflammatory diseases
Interleukin 13
Interleukin 4
Interleukin 5
Iridoid Glucosides - chemistry
Iridoid Glucosides - isolation & purification
Iridoid Glucosides - pharmacology
Laboratory animals
Lung - immunology
Lung - pathology
Lungs
Lymphocytes
Lymphocytes T
Medicine
Medicine and Health Sciences
Mice
Mice, Inbred BALB C
Mucus
Pharmacy
Pyroglyphidae
Respiratory tract
Respiratory tract diseases
Th1 Cells - immunology
Th1 Cells - pathology
Th2 Cells - immunology
Th2 Cells - pathology
Tissue analysis
Transcription factors
γ-Interferon
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Summary:Picroside II isolated from Pseudolysimachion rotundum var. subintegrum has been used as traditional medicine to treat inflammatory diseases. In this study, we assessed whether picroside II has inhibitory effects on airway inflammation in a mouse model of house dust mite (HDM)-induced asthma. In the HDM-induced asthmatic model, picroside II significantly reduced inflammatory cell counts in the bronchoalveolar lavage fluid (BALF), the levels of total immunoglobulin (Ig) E and HDM-specific IgE and IgG1 in serum, airway inflammation, and mucus hypersecretion in the lung tissues. ELISA analysis showed that picroside II down-regulated the levels of Th2-related cytokines (including IL-4, IL-5, and IL-13) and asthma-related mediators, but it up-regulated Th1-related cytokine, IFNγ in BALF. Picroside II also inhibited the expression of Th2 type cytokine genes and the transcription factor GATA3 in the lung tissues of HDM-induced mice. Finally, we demonstrated that picroside II significantly decreased the expression of GATA3 and Th2 cytokines in developing Th2 cells, consistent with in vivo results. Taken together, these results indicate that picroside II has protective effects on allergic asthma by reducing GATA3 expression and Th2 cytokine bias.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0167098