Transcriptomes of Trypanosoma brucei rhodesiense from sleeping sickness patients, rodents and culture: Effects of strain, growth conditions and RNA preparation methods

All of our current knowledge of African trypanosome metabolism is based on results from trypanosomes grown in culture or in rodents. Drugs against sleeping sickness must however treat trypanosomes in humans. We here compare the transcriptomes of Trypanosoma brucei rhodesiense from the blood and cere...

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Bibliographic Details
Published in:PLoS neglected tropical diseases 2018-02, Vol.12 (2), p.e0006280-e0006280
Main Authors: Mulindwa, Julius, Leiss, Kevin, Ibberson, David, Kamanyi Marucha, Kevin, Helbig, Claudia, Melo do Nascimento, Larissa, Silvester, Eleanor, Matthews, Keith, Matovu, Enock, Enyaru, John, Clayton, Christine
Format: Article
Language:English
Subjects:
African trypanosomiasis
Analysis
Animals
Bacteriological Techniques - methods
Biochemistry
Biology and Life Sciences
Blood
Blood culture
Bovidae
Cerebrospinal fluid
Culture
Data processing
Disease
DNA, Kinetoplast - genetics
Fluids
Funding
Gene expression
Gene Expression Profiling
Genetic aspects
Growth conditions
Humans
Immunology
Kinases
Laboratories
Medicine and Health Sciences
Metabolism
Methods
Molecular biology
Nucleic acids
Parasites
Parasitology
Patients
Poly(A)
Polyadenine
Protein kinase
Protein Kinases - genetics
Proteins
Protozoa
Protozoan Proteins - genetics
Rats
Research and analysis methods
Ribonucleic acid
RNA
RNA sequencing
RNA, Messenger - genetics
RNA, Protozoan - genetics
RNA, Protozoan - isolation & purification
RNA-binding protein
RNA-Binding Proteins - genetics
Rodentia
Rodentia - parasitology
Rodents
Sport science
Strain
Transcription (Genetics)
Transcriptome
Tropical diseases
Trypanosoma brucei
Trypanosoma brucei rhodesiense - genetics
Trypanosoma brucei rhodesiense - growth & development
Trypanosoma brucei rhodesiense - isolation & purification
Trypanosoma brucei rhodesiense - metabolism
Trypanosome
Trypanosomiasis, African - blood
Trypanosomiasis, African - cerebrospinal fluid
Trypanosomiasis, African - parasitology
Vector-borne diseases
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Description
Summary:All of our current knowledge of African trypanosome metabolism is based on results from trypanosomes grown in culture or in rodents. Drugs against sleeping sickness must however treat trypanosomes in humans. We here compare the transcriptomes of Trypanosoma brucei rhodesiense from the blood and cerebrospinal fluid of human patients with those of trypanosomes from culture and rodents. The data were aligned and analysed using new user-friendly applications designed for Kinetoplastid RNA-Seq data. The transcriptomes of trypanosomes from human blood and cerebrospinal fluid did not predict major metabolic differences that might affect drug susceptibility. Usefully, there were relatively few differences between the transcriptomes of trypanosomes from patients and those of similar trypanosomes grown in rats. Transcriptomes of monomorphic laboratory-adapted parasites grown in in vitro culture closely resembled those of the human parasites, but some differences were seen. In poly(A)-selected mRNA transcriptomes, mRNAs encoding some protein kinases and RNA-binding proteins were under-represented relative to mRNA that had not been poly(A) selected; further investigation revealed that the selection tends to result in loss of longer mRNAs.
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0006280