Intracellular trafficking of begomoviruses in the midgut cells of their insect vector

Begomoviruses are exclusively transmitted by whiteflies in a persistent circulative manner and cause considerable economic losses to crop production worldwide. Previous studies have shown that begomoviruses accumulate in vesicle-like structures in whitefly midgut cells and that clathrin-mediated end...

Full description

Saved in:
Bibliographic Details
Published in:PLoS pathogens 2018-01, Vol.14 (1), p.e1006866-e1006866
Main Authors: Xia, Wen-Qiang, Liang, Yan, Chi, Yao, Pan, Li-Long, Zhao, Jing, Liu, Shu-Sheng, Wang, Xiao-Wei
Format: Article
Language:English
Subjects:
Agricultural economics
Agricultural production
Agrobacterium tumefaciens
Analysis
Animals
Autophagy
Begomovirus - metabolism
Bemisia tabaci
Biological Transport
Biology and Life Sciences
Clathrin
Crop production
Crops
Digestive System - virology
Disease transmission
Economic impact
Endocytosis
Endoplasmic reticulum
Endosomes
Flowers & plants
Funding
Golgi apparatus
Hemiptera - metabolism
Hemiptera - virology
Hemolymph
Insect Vectors - virology
Insects
Internalization
Intracellular
Laboratories
Lysosomes
Medicine and Health Sciences
Midgut
Molecular biology
Nexin
Pathogens
Plant diseases
Protein turnover
Ribonucleic acid
RNA
RNA-mediated interference
Solanum lycopersicum - virology
Tomatoes
Transport
Viruses
Whiteflies
Yellow leaf
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Begomoviruses are exclusively transmitted by whiteflies in a persistent circulative manner and cause considerable economic losses to crop production worldwide. Previous studies have shown that begomoviruses accumulate in vesicle-like structures in whitefly midgut cells and that clathrin-mediated endocytosis is responsible for their internalization. However, the process by which begomoviruses are trafficked within whitefly midgut cells remains largely unknown. In this study, we investigated the roles of vesicle trafficking in the transport of Tomato yellow leaf curl virus (TYLCV), a begomovirus that has spread to over 50 countries and caused extensive damage to a range of important crops, within midgut cells of whitefly (Bemisia tabaci). By disrupting vesicle trafficking using RNA silencing and inhibitors, we demonstrated that the early steps of endosomal trafficking are important for the intracellular transport of TYLCV in the whitefly midgut. In addition, our data show that, unlike many animal viruses, TYCLV is trafficked within cells in a manner independent of recycling endosomes, late endosomes, lysosomes, the Golgi apparatus and the endoplasmic reticulum. Instead, our results suggest that TYLCV might be transported directly from early endosomes to the basal plasma membrane and released into the hemolymph. Silencing of the sorting nexin Snx12, which may be involved in membrane tubulation, resulted in fewer viral particles in hemolymph; this suggests that the tubular endosomal network may be involved in the transport of TYLCV. Our results also support a role for the endo-lysosomal system in viral degradation. We further showed that the functions of vector early endosomes and sorting nexin Snx12 are conserved in the transmission of several other begomoviruses. Overall, our data indicate the importance of early endosomes and the tubular endosomal network in begomovirus transmission.
ISSN:1553-7374
1553-7366
1553-7374
DOI:10.1371/journal.ppat.1006866