Cell type-specific suppression of mechanosensitive genes by audible sound stimulation

Audible sound is a ubiquitous environmental factor in nature that transmits oscillatory compressional pressure through the substances. To investigate the property of the sound as a mechanical stimulus for cells, an experimental system was set up using 94.0 dB sound which transmits approximately 10 m...

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Bibliographic Details
Published in:PloS one 2018-01, Vol.13 (1), p.e0188764-e0188764
Main Authors: Kumeta, Masahiro, Takahashi, Daiji, Takeyasu, Kunio, Yoshimura, Shige H
Format: Article
Language:English
Subjects:
Acoustics
Analysis
Biology and Life Sciences
Cavitation
Cell culture
Cells (Biology)
Engineering and Technology
Environmental factors
Experiments
Genes
Growth factors
Kinases
Mechanotransduction
Myoblasts
Neuroblastoma
Neuroblastoma cells
Physical Sciences
Pressure
Research and analysis methods
Ribonucleic acid
RNA
Sound
Stimulation
Stimuli (Psychology)
Stromal cells
Transcription
Ultrasonic imaging
Ultrasound
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Summary:Audible sound is a ubiquitous environmental factor in nature that transmits oscillatory compressional pressure through the substances. To investigate the property of the sound as a mechanical stimulus for cells, an experimental system was set up using 94.0 dB sound which transmits approximately 10 mPa pressure to the cultured cells. Based on research on mechanotransduction and ultrasound effects on cells, gene responses to the audible sound stimulation were analyzed by varying several sound parameters: frequency, wave form, composition, and exposure time. Real-time quantitative PCR analyses revealed a distinct suppressive effect for several mechanosensitive and ultrasound-sensitive genes that were triggered by sounds. The effect was clearly observed in a wave form- and pressure level-specific manner, rather than the frequency, and persisted for several hours. At least two mechanisms are likely to be involved in this sound response: transcriptional control and RNA degradation. ST2 stromal cells and C2C12 myoblasts exhibited a robust response, whereas NIH3T3 cells were partially and NB2a neuroblastoma cells were completely insensitive, suggesting a cell type-specific response to sound. These findings reveal a cell-level systematic response to audible sound and uncover novel relationships between life and sound.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0188764